Description

[3'-13C]Thymidine CAS NO 478511-06-7 is a stable isotope-labeled nucleoside analog, where the carbon-13 isotope is specifically incorporated at the 3' position of the deoxyribose sugar. This precise labeling is critical for advanced research requiring high sensitivity and specificity in metabolic pathway tracing. It is an essential tool for scientists in pharmaceutical R&D, clinical diagnostics, and academic research focused on DNA synthesis, cell proliferation studies, and the development of novel antiviral or anticancer agents.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to study the dynamics of nucleotide metabolism and DNA synthesis pathways in living cells.
• Drug Mechanism of Action Studies: Employed in research to elucidate the metabolic pathways and cellular uptake of nucleoside-based pharmaceuticals.
• Biomarker Discovery & Clinical Diagnostics: Serves as an internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of endogenous nucleosides in biological samples.
• Antiviral & Anticancer Research: Utilized in the development and testing of novel therapeutic agents that target DNA replication, such as antiviral drugs and chemotherapeutics.
• Stable Isotope Probing (SIP): Applied in microbiological and environmental studies to track the incorporation of labeled thymidine into microbial DNA, identifying active populations.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: The 13C label provides a non-radioactive, sensitive probe for structural and dynamic studies of nucleic acids and their interactions.

Basic Information

Product Name	[3'-13C]Thymidine
CAS No.	478511-06-7
Molecular Formula	C10H14N2O5 (with 13C at specified position)
Molecular Weight	Contact for details
Synonyms	1-(2-Deoxy-β-D-erythro-pentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione-3'-13C; Thymidine-3'-13C; 2'-Deoxythymidine-3'-13C; dT-3'-13C; 1-(2-Deoxy-β-D-ribofuranosyl)thymine-3'-13C; 5-Methyldeoxyuridine-3'-13C; Stable Isotope Labeled Thymidine
EINECS	Contact for details

Quality Control

Every batch of [3'-13C]Thymidine is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing to ensure batch-to-batch consistency for research reproducibility. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting key parameters such as isotopic enrichment, chemical purity, and absence of biological contaminants.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Enrichment (NMR/MS)	≥ 99 atom % 13C
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Biological Contamination (LAL)	< 0.25 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.