Description

5-Methyl-[3'-13C]Uridine is a stable isotope-labeled nucleoside analog, specifically labeled with carbon-13 at the 3' position of the ribose ring. This precise isotopic labeling is critical for advanced research applications requiring high sensitivity and specificity in detection and quantification. It is an essential building block for researchers in pharmaceutical development, metabolic pathway analysis, and diagnostic probe synthesis, where tracing molecular fate and understanding biochemical mechanisms are paramount.

Application

• Isotope-Labeled Internal Standards for the quantitative analysis of nucleosides and nucleotides in biological samples using LC-MS/MS.
• Metabolic Flux Analysis (MFA) to study the pathways and rates of cellular metabolism in cancer research and systems biology.
• Tracer Studies in Drug Metabolism and Pharmacokinetics (DMPK) to track the absorption, distribution, metabolism, and excretion of novel therapeutic nucleoside analogs.
• Synthesis of Labeled RNA Probes for use in structural biology studies, such as NMR spectroscopy, to investigate RNA conformation and dynamics.
• Biomarker Discovery and Validation, serving as a reference compound in the development of clinical diagnostic assays.
• Substrate for Enzymology Studies to elucidate the mechanism and kinetics of methyltransferases and other RNA-modifying enzymes.

Basic Information

Product Name	5-Methyl-[3'-13C]Uridine
CAS No.	478511-00-1
Molecular Formula	C10H1413CN2O6
Molecular Weight	260.20 g/mol (for 13C1 isotopologue)
Synonyms	3'-13C-5-Methyluridine; [3'-13C]-5-Methyluridine; 5-Methyluridine-3'-13C; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-((13C)hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione; m5U-3'-13C; Labeled 5-Methyluridine
EINECS	Contact for details

Quality Control

Every batch of 5-Methyl-[3'-13C]Uridine is manufactured and tested under strict quality management systems. We guarantee high chemical purity and isotopic enrichment, supported by comprehensive analytical data including NMR, HPLC, and MS. Certificates of Analysis (COA) detailing purity, isotopic purity (atom % 13C), and specific analytical results are provided with each shipment to ensure compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % 13C)	≥ 99%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.