Description

[1',2',3',4',5'-13C5]2'-Deoxyuridine is a stable isotope-labeled analog of the nucleoside 2'-deoxyuridine, where five carbon atoms are enriched with the carbon-13 isotope. This compound is essential as a critical internal standard and tracer in quantitative mass spectrometry-based metabolomics and pharmacokinetic studies, ensuring unparalleled accuracy in measurement. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and academic institutions engaged in advanced drug metabolism, cancer research, and nucleic acid biochemistry.

Application

• Quantitative Mass Spectrometry (MS) Internal Standard: Used as a certified reference material for the precise quantification of endogenous 2'-deoxyuridine and related metabolites in biological samples.
• Drug Metabolism and Pharmacokinetics (DMPK): Serves as a stable isotope tracer to study the absorption, distribution, metabolism, and excretion (ADME) profiles of nucleoside-based pharmaceuticals.
• Cancer and Cell Proliferation Research: Employed in tracing DNA synthesis and turnover in cell cultures and model organisms to investigate mechanisms of oncogenesis and therapeutic response.
• Metabolomics and Flux Analysis: Enables accurate mapping of metabolic pathways, particularly in pyrimidine metabolism, by providing a non-radioactive, MS-detectable tracer.
• Diagnostic Assay Development: Utilized in the calibration and validation of high-sensitivity LC-MS/MS kits for clinical diagnostics related to mitochondrial disorders or chemotherapy monitoring.
• Quality Control for Pharmaceutical Manufacturing: Acts as a reference standard in the purity and potency testing of nucleoside analog active pharmaceutical ingredients (APIs).

Basic Information

Product Name	[1',2',3',4',5'-13C5]2'-Deoxyuridine
CAS No.	478510-94-0
Molecular Formula	C9[13C]5H14N2O5
Molecular Weight	~257.19 g/mol (for 13C5 labeled form)
Synonyms	2'-Deoxyuridine-13C5; 1-(2-Deoxy-β-D-ribofuranosyl)uracil-13C5; dU-13C5; 2'-Deoxyuridine (13C5); Uridine, 2'-deoxy-, 13C-labeled; 2'-Deoxyuridine-1',2',3',4',5'-13C5; Thymidine precursor-13C5; 1-(2-Deoxyribosyl)uracil-13C5
EINECS	Contact for details

Quality Control

Every batch of [1',2',3',4',5'-13C5]2'-Deoxyuridine is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, with comprehensive testing via NMR (1H, 13C), HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (HPLC), isotopic enrichment, and absence of impurities. Our quality commitment supports compliance with the rigorous standards required for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C (at labeled positions)
Chemical Purity (NMR)	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.