Description

[5'-13C]2'-Deoxyuridine CAS NO 478510-91-7 is a stable isotope-labeled nucleoside analog, specifically deuterated at the 5' carbon position. This compound is essential for advanced research requiring precise metabolic and pharmacokinetic tracing in nucleic acid studies. It is a critical reagent for scientists in pharmaceutical R&D, academic biochemistry, and diagnostic development who require high-purity, isotopically enriched standards for mass spectrometry and NMR-based assays.

Application

• Metabolic Pathway Tracing: Used as an internal standard in LC-MS and GC-MS to study the metabolism and catabolism of nucleosides and nucleotides.
• Drug Development & Pharmacokinetics (PK/PD): Serves as a labeled analog in the research and quantification of antiviral and anticancer nucleoside drugs.
• Biomarker Discovery & Validation: Enables accurate quantification of deoxyuridine and related metabolites in biological fluids for diagnostic assay development.
• NMR Spectroscopy: Provides a non-radioactive tracer for real-time monitoring of DNA/RNA synthesis and repair mechanisms in cell culture and in vitro systems.
• Stable Isotope Labeling in Cell Culture (SILAC) & Proteomics: Can be incorporated into nucleic acid precursors for studying cell proliferation and cycle dynamics.
• Quality Control Reference Standard: Acts as a certified reference material (CRM) for calibrating analytical instruments in quality control laboratories.

Basic Information

Product Name	[5'-13C]2'-Deoxyuridine
CAS No.	478510-91-7
Molecular Formula	C9H1213CN2O5
Molecular Weight	Contact for details
Synonyms	1-((2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methyl-1H-pyrimidine-2,4-dione-5'-13C; 2'-Deoxy-5-methyluridine-5'-13C; 5'-13C-Thymidine deoxyriboside; 5'-13C-dT; 5'-Carbon-13 labeled 2'-deoxyuridine; Isotopically labeled deoxyuridine; [5'-13C]dU; Stable isotope-labeled nucleoside
EINECS	Contact for details

Quality Control

Every batch of [5'-13C]2'-Deoxyuridine is manufactured and analyzed under strict quality management systems. We provide comprehensive Certificates of Analysis (COA) detailing isotopic purity (e.g., >98 atom % 13C), chemical purity (by HPLC), and identity confirmation (by NMR and MS). Our quality commitment ensures the material meets the stringent requirements for use as a reference standard in regulated research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, please contact our technical team for specific recommendations.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (NMR/MS)	≥98 atom % 13C
Chemical Purity (by HPLC)	≥95.0%
Water Content (KF)	≤2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.