Description

[2'-13C]2'-Deoxyuridine is a stable isotope-labeled nucleoside derivative, specifically deuterated at the 2' carbon position. This precise isotopic labeling is critical for applications in quantitative mass spectrometry, metabolic flux analysis, and pharmacokinetic studies, where it serves as an internal standard to ensure accurate and reliable data. It is an essential research tool for scientists in pharmaceutical development, clinical diagnostics, and biochemistry who require high-purity, well-characterized reference materials for advanced analytical techniques.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the accurate measurement of endogenous nucleosides and related metabolites.
• Metabolic Pathway Tracing: Employed in 13C metabolic flux analysis (MFA) to study nucleotide synthesis, DNA repair mechanisms, and cellular metabolism in cancer research and systems biology.
• Drug Metabolism and Pharmacokinetics (DMPK): Serves as a stable isotope-labeled analog in ADME (Absorption, Distribution, Metabolism, and Excretion) studies for novel antiviral or anticancer nucleoside analogs.
• Biomarker Discovery and Validation: Facilitates the precise quantification of modified nucleosides in biological fluids, aiding in the discovery of potential disease biomarkers.
• Nucleic Acid Research: Used in mechanistic studies of enzyme kinetics, particularly for thymidylate synthase and other enzymes involved in the pyrimidine salvage pathway.
• Quality Control in Diagnostics: Acts as a calibrant or control material in the development and validation of clinical diagnostic assays for genetic or metabolic disorders.

Basic Information

Product Name	[2'-13C]2'-Deoxyuridine
CAS No.	478510-87-1
Molecular Formula	C9H1213CN2O5
Molecular Weight	Contact for details
Synonyms	2'-Deoxyuridine-2'-13C; 1-(2-Deoxy-2-13C-β-D-erythro-pentofuranosyl)uracil; 2'-13C-2'-Deoxyuridine; dU-2'-13C; Stable Isotope Labeled 2'-Deoxyuridine; [2'-13C]dU
EINECS	Contact for details

Quality Control

Every batch of [2'-13C]2'-Deoxyuridine is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data including isotopic purity (enrichment) by NMR and/or MS, chemical purity by HPLC, and identity confirmation. A detailed Certificate of Analysis (COA) specifying batch-specific results for all critical parameters is available with each shipment to ensure traceability and compliance with your research or quality protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.