Description

5-Pyrimidinecarboxylic Acid, 4-Amino-2-Methyl-, Labeled With Nitrogen-15 (9Ci) is a stable isotope-labeled derivative of a key pyrimidine carboxylic acid building block. This compound is essential for advanced research and development, providing a non-radioactive tracer for mechanistic studies, metabolic pathway analysis, and quantitative mass spectrometry. It is primarily utilized by pharmaceutical, agrochemical, and academic research institutions engaged in the synthesis of labeled compounds, drug discovery, and proteomics research.

Application

• Isotope-Labeled Standard for Mass Spectrometry (MS): Used as an internal standard for the precise quantification of pyrimidine-based metabolites or drug compounds in complex biological matrices via LC-MS/MS and GC-MS.
• Metabolic Pathway Tracing: Employed in 15N NMR spectroscopy and MS-based studies to elucidate biosynthetic pathways, degradation routes, and the metabolic fate of pyrimidine derivatives.
• Pharmaceutical Research & Development: Serves as a critical building block for synthesizing nitrogen-15 labeled drug candidates, enabling detailed pharmacokinetic (PK), pharmacodynamic (PD), and absorption, distribution, metabolism, and excretion (ADME) studies.
• Proteomics and Biomarker Discovery: Can be incorporated into stable isotope labeling by amino acids in cell culture (SILAC) or other labeling strategies for quantitative proteomic analysis.
• Agrochemical Research: Used in the development and environmental fate studies of novel pyrimidine-based herbicides, fungicides, or plant growth regulators.
• Chemical Mechanism Studies: Aids in investigating reaction mechanisms, particularly those involving nitrogen transfer or rearrangement, in synthetic organic chemistry.

Basic Information

Product Name	5-Pyrimidinecarboxylic Acid, 4-Amino-2-Methyl-, Labeled With Nitrogen-15 (9Ci)
CAS No.	41279-10-1
Molecular Formula	C6H715N3O2
Molecular Weight	154.12 g/mol (for 15N labeled form)
Synonyms	4-Amino-2-methylpyrimidine-5-carboxylic acid (15N); 2-Methyl-4-aminopyrimidine-5-carboxylic acid-15N; 4-Amino-2-methyl-5-pyrimidinecarboxylic acid (Nitrogen-15); 5-Carboxy-2-methyl-4-aminopyrimidine (15N); Vitamin B1 intermediate analog-15N; 2-Methyl-4-amino-5-pyrimidinecarboxylic acid-15N; 4-Amino-2-methyl-5-pyrimidinecarboxylic-15N acid; (15N)-4-Amino-2-methylpyrimidine-5-carboxylic acid
EINECS	Contact for details

Quality Control

Every batch of our isotope-labeled compounds is produced and analyzed under strict quality protocols to ensure high chemical and isotopic purity, critical for sensitive analytical applications. Our standard quality commitment includes verification of structure (NMR, MS), isotopic enrichment (typically ≥98 atom % 15N), and chemical purity (by HPLC). A comprehensive Certificate of Analysis (COA) detailing all specifications, including batch-specific isotopic enrichment and chromatographic data, is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation due to moisture absorption. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
15N Isotopic Enrichment	≥98 atom %
Assay (HPLC)	≥95%
Water Content (KF)	≤2.0%
Residue on Ignition	≤0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.