Description

S-Adenosyl-L-Methionine, [Methyl-14C] CAS NO 4989-98-4 is a radiolabeled derivative of the critical biological cofactor S-adenosylmethionine (SAMe), where the methyl group is tagged with carbon-14. This compound is an indispensable tool for researchers studying methylation processes, metabolic pathways, and enzyme kinetics in biochemical and pharmacological research. It is primarily used by scientists in pharmaceutical R&D, academic institutions, and contract research organizations (CROs) for tracer studies, drug metabolism investigations, and detailed mechanistic analysis.

Application

• Biochemical Tracer Studies: Used to track and quantify methyl group transfer in enzymatic reactions, such as those catalyzed by methyltransferases.
• Drug Metabolism and Pharmacokinetics (DMPK): Essential for investigating the metabolic fate of new drug candidates, specifically pathways involving transmethylation.
• Enzyme Mechanism & Kinetics: Employed to elucidate the catalytic mechanisms of SAM-dependent enzymes, including determination of kinetic parameters.
• Epigenetics Research: A key reagent for studying DNA and histone methylation, which are central processes in epigenetic regulation.
• Metabolic Pathway Analysis: Facilitates the mapping of biosynthetic and catabolic pathways in cells and tissues where SAMe is a substrate.
• Radiochemical Synthesis: Serves as a labeled precursor for the synthesis of other carbon-14 labeled compounds for research purposes.

Basic Information

Product Name	S-Adenosyl-L-Methionine, [Methyl-14C]
CAS No.	4989-98-4
Molecular Formula	C15H23N6O5S+ (with 14C label)
Molecular Weight	~399.4 g/mol (unlabeled)
Synonyms	S-Adenosylmethionine-methyl-14C; SAMe-[methyl-14C]; AdoMet-[methyl-14C]; (3S)-5'-[(3-Amino-3-carboxypropyl)methylsulfonio]-5'-deoxyadenosine, [methyl-14C]; 14C-SAM; Radiolabeled SAM; Carbon-14 Labeled S-Adenosylmethionine; S-Adenosyl-L-Methionine (14C-methyl)
EINECS	For unlabeled form: 225-625-8. Contact for specific radiolabeled product details.

Quality Control

Every batch of our radiolabeled S-Adenosyl-L-Methionine, [Methyl-14C] is produced and tested under stringent conditions to ensure high chemical and radiochemical purity, specific activity, and stability. Quality assurance protocols include rigorous analytical testing via HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing purity, specific activity (mCi/mmol or GBq/mmol), concentration, solvent, and analytical chromatograms. Our quality system is designed to meet the exacting standards of research and regulatory support studies.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C or as specified on the product label and COA. The product is light-sensitive (store away from light). Allow to reach ambient temperature before opening to prevent condensation. Store in accordance with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Solution (color may vary)
Identification (HPLC)	Conforms to reference standard
Chemical Purity (HPLC)	≥ 95%
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	50-60 mCi/mmol (1.85-2.22 GBq/mmol) [Contact for exact value]
Concentration	Specified per batch on COA
Solvent	Typically dilute acid or aqueous ethanol (specified on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.