Description

Uridine-2-13C, 1,3-15N2 is a stable isotope-labeled nucleoside, specifically enriched with carbon-13 at the 2-position and nitrogen-15 at the 1 and 3 positions of the uracil ring. This compound is essential as a critical internal standard and tracer in quantitative mass spectrometry-based metabolomics and pharmacokinetic studies, ensuring unparalleled analytical accuracy. It is primarily utilized by research institutions, pharmaceutical companies, and CROs (Contract Research Organizations) engaged in advanced drug development, metabolic pathway analysis, and biomarker discovery.

Application

• Quantitative Mass Spectrometry (MS): Serves as an internal standard for the precise quantification of endogenous uridine and related metabolites in complex biological matrices like plasma, urine, and tissue extracts.
• Drug Metabolism and Pharmacokinetics (DMPK): Used as a tracer to study the absorption, distribution, metabolism, and excretion (ADME) of nucleoside-based pharmaceuticals.
• Metabolic Flux Analysis (MFA): Enables the tracing of carbon and nitrogen through nucleotide salvage and de novo synthesis pathways in cell culture and in vivo models.
• Clinical Diagnostics & Biomarker Research: Facilitates the accurate measurement of uridine levels, which can be indicators of certain metabolic disorders or cellular stress.
• NMR Spectroscopy: Provides a non-radioactive, stable isotope probe for structural and dynamic studies of RNA and nucleoside-interacting proteins.
• Pharmaceutical Reference Standard: Acts a certified reference material (CRM) for quality control and method validation in analytical laboratories.

Basic Information

Product Name	Uridine-2-13C, 1,3-15N2
CAS No.	369656-75-7
Molecular Formula	C9H1213CN215N2O6
Molecular Weight	Contact for details
Synonyms	1-β-D-Ribofuranosyl-2-13C-1,3-15N2-uracil; [2-13C,1,3-15N2]Uridine; Uridine-2-13C,1,3-15N2; Isotopically Labeled Uridine; 13C,15N2-Uridine; Stable Isotope-Labeled Uridine; Uridine (2-13C, 1,3-15N2)
EINECS	Contact for details

Quality Control

Every batch of Uridine-2-13C, 1,3-15N2 is manufactured and analyzed under strict quality systems to ensure identity, purity, and isotopic enrichment meet the highest standards for research use. Our products undergo rigorous quality testing, including NMR and LC-MS, to ensure compliance with specifications. Certificates of Analysis (COA) detailing batch-specific data for isotopic purity, chemical purity, and analytical methods are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (13C)	≥ 99 atom % 13C
Isotopic Purity (15N)	≥ 98 atom % 15N
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.