Description

Dar-4Mt CAS NO 339527-82-1 is a specialized chemical compound, a derivative of 4-methylumbelliferone, widely recognized for its utility as a fluorogenic substrate and a key intermediate in advanced biochemical research and synthesis. This compound matters for its high sensitivity and specificity in enzymatic assays, particularly for detecting protease and phosphatase activity, which is critical for drug discovery and diagnostic development. It is essential for research scientists, pharmaceutical developers, and diagnostic manufacturers requiring reliable, high-purity biochemical tools.

Application

• Fluorogenic Substrate for Enzymatic Assays: Primarily used as a sensitive substrate for detecting and quantifying the activity of various enzymes, including proteases, phosphatases, and sulfatases in high-throughput screening (HTS).
• Biochemical Research & Diagnostics: Serves as a critical reagent in developing research kits and diagnostic assays for studying cellular processes, disease biomarkers, and signal transduction pathways.
• Pharmaceutical Intermediate: Acts as a key building block in the synthesis of more complex fluorogenic probes and pharmaceutical candidates targeting specific enzymatic pathways.
• Cell-Based Imaging: Utilized in live-cell imaging applications to visualize and monitor enzymatic activity in real-time within biological systems.
• Quality Control in Biomanufacturing: Employed in purity and activity testing of enzyme preparations used in biotechnological and therapeutic protein production.

Basic Information

Product Name	Dar-4Mt
CAS No.	339527-82-1
Molecular Formula	C12H9NO4
Molecular Weight	231.20 g/mol
Synonyms	7-Amino-4-methylcoumarin-3-acetic acid; AMCA; 3-Carboxymethyl-7-amino-4-methylcoumarin; 7-Amino-4-methyl-2-oxo-2H-chromene-3-acetic acid; 7-Amino-4-methyl-3-carboxymethylcoumarin; Dar-4M; AMC-acetic acid; 3-(Carboxymethyl)-7-amino-4-methyl-2H-chromen-2-one
EINECS	Contact for details

Quality Control

Our Dar-4Mt is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under an inert atmosphere to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	Off-white to pale yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Fluorescence Properties	Ex/Em ~345/445 nm (pH dependent)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.