Description

Cholesterol-25,26,27-13C3 is a stable isotope-labeled analog of cholesterol, where three carbon atoms at positions 25, 26, and 27 are replaced with the 13C isotope. This compound is essential as an internal standard in quantitative mass spectrometry, enabling precise and accurate measurement of cholesterol and its metabolites in complex biological matrices. It is a critical reagent for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and nutritional science industries.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a high-purity internal standard for the absolute quantification of cholesterol in bioanalytical assays.
• Clinical Research & Diagnostics: Used in method development and validation for measuring cholesterol levels in serum, plasma, and tissues to study metabolic disorders like dyslipidemia.
• Pharmaceutical Development: Employed in pharmacokinetic and pharmacodynamic studies to track cholesterol absorption, synthesis, and metabolism during drug efficacy testing.
• Nutritional & Food Science: Facilitates the accurate analysis of cholesterol content and bioavailability in fortified foods and dietary supplements.
• Metabolic Pathway Tracing: Acts as a tracer in isotope dilution studies to elucidate cholesterol biosynthesis and turnover rates in cellular and animal models.
• Reference Standard Manufacturing: Used by certified reference material (CRM) producers to create calibrated standards for laboratory proficiency testing.

Basic Information

Product Name	Cholesterol-25,26,27-13C3
CAS No.	335080-97-2
Molecular Formula	C₂₇[13C]₃H₄₆O
Molecular Weight	~390.67 g/mol (exact value depends on isotopic enrichment)
Synonyms	(5-Cholesten-3β-ol-25,26,27-13C3); 13C3-Labeled Cholesterol; Cholesterol (25,26,27-13C3); [25,26,27-13C3]Cholesterol; Isotopic Cholesterol Internal Standard; 13C3-Cholesterol; Stable Isotope-Labeled Cholesterol; Cholesterol-13C3
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-25,26,27-13C3 is manufactured and analyzed under strict quality management systems. Our products undergo rigorous quality testing to ensure compliance with industry standards for isotopic purity, chemical purity, and stability. Certificates of Analysis (COA) detailing isotopic enrichment (typically ≥99 atom % 13C), chemical purity (by HPLC), and specific analytical data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as indicated on the label or COA. For long-term storage, consider storing desiccated at -20°C. Handle and store in accordance with good laboratory practices.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR / NMR)	Conforms to structure
Isotopic Purity (Atom % 13C)	≥ 99% (at positions 25,26,27)
Chemical Purity (HPLC)	≥ 98%
Specific Rotation	Contact for details
Solubility	Soluble in organic solvents (e.g., chloroform, ethanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.