Description

Cholesterol-3-13C is a stable isotope-labeled analog of cholesterol, where a carbon-13 atom is incorporated at the 3-position of the sterol backbone. This precise labeling is critical for advanced analytical and metabolic research, enabling highly accurate quantification and pathway tracing. It is an essential tool for researchers and developers in the pharmaceutical, diagnostic, and life science sectors who require definitive standards for mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.

Application

• Internal Standard for Mass Spectrometry: Serves as a critical quantitative reference in LC-MS and GC-MS analyses of cholesterol and related sterols in biological matrices.
• Metabolic Pathway Tracing: Used in in vitro and in vivo studies to elucidate cholesterol biosynthesis, absorption, and turnover using 13C-NMR or MS detection.
• Pharmaceutical Research & Development: Employed in the development and validation of assays for lipid-lowering drugs and in studying drug-sterol interactions.
• Clinical Diagnostics: Enhances the accuracy of diagnostic tests for lipid disorders, such as hypercholesterolemia, by providing a definitive isotopic standard.
• Biochemical Research: Facilitates studies on membrane biophysics, lipid raft formation, and steroid hormone synthesis where precise cholesterol tracking is required.
• Food & Nutritional Science: Applied in research on cholesterol metabolism from dietary sources and the efficacy of functional foods or nutraceuticals.

Basic Information

Product Name	Cholesterol-3-13C
CAS No.	334974-05-9
Molecular Formula	C₂₇[13C]H₄₆O
Molecular Weight	387.66 g/mol (approx.)
Synonyms	(3β)-Cholest-5-en-3-ol-3-13C; 13C Labeled Cholesterol; Cholesterol (3-13C); [3-13C]-Cholesterol; 5-Cholesten-3β-ol-3-13C; Isotopic Cholesterol; Cholesterol-13C Internal Standard; Stable Isotope-Labeled Cholesterol
EINECS	Contact for details

Quality Control

Every batch of Cholesterol-3-13C is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Our products undergo rigorous quality testing, including isotopic enrichment analysis by NMR and MS, and chemical purity assessment by HPLC. Certificates of Analysis (COA) are provided, detailing the isotopic purity, chemical purity, and identity confirmation. We support compliance with relevant GLP and ISO standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as specified on the label or COA. For long-term storage, consider keeping the product under an inert atmosphere at -20°C to maximize stability. The compound is light-sensitive and should be handled accordingly.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR / NMR)	Conforms to structure
Isotopic Purity (13C)	≥ 99 atom % 13C
Chemical Purity (HPLC)	≥ 98.0%
Specific Rotation	-34° to -38° (c=2, in dioxane)
Melting Point	147°C - 150°C
Solubility	Soluble in organic solvents (e.g., chloroform, ethanol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.