Description

n-(Deoxyguanosin-8-Yl)-2-Amino-1-Methyl-D3-6-Phenylimidazo[4,5-B]Pyridine is a stable isotope-labeled (deuterated) metabolite of the heterocyclic aromatic amine, PhIP. This compound is of critical importance for researchers as a high-purity analytical standard for the accurate quantification and metabolic profiling of PhIP-DNA adducts. It is primarily needed by analytical laboratories, toxicology research institutions, and pharmaceutical companies engaged in advanced studies of food-borne carcinogens, DNA damage mechanisms, and biomarker discovery.

Application

• Analytical Reference Standard: For use as a primary standard in LC-MS/MS, HPLC-UV, and other chromatographic methods for the precise quantification of PhIP-DNA adducts in biological samples.
• Metabolic and Toxicological Research: Enables detailed investigation into the metabolic activation pathways of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and its binding to DNA.
• Biomarker Development: Serves as a critical calibrant in the development and validation of sensitive assays for detecting DNA adducts as biomarkers of exposure to dietary mutagens.
• Carcinogenesis Studies: Used in mechanistic studies to understand the role of specific DNA adducts in mutation and cancer initiation, particularly related to cooked meat consumption.
• Quality Control and Method Validation: Essential for ensuring the accuracy and reliability of internal laboratory testing protocols in food safety and environmental health sectors.

Basic Information

Product Name	n-(Deoxyguanosin-8-Yl)-2-Amino-1-Methyl-D3-6-Phenylimidazo[4,5-B]Pyridine
CAS No.	303173-39-9
Molecular Formula	C24H23D3N8O4
Molecular Weight	481.52 g/mol
Synonyms	N-(Deoxyguanosin-8-yl)-PhIP-d3; 8-(Deoxyguanosin-N²-yl)-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-d3; PhIP-DNA Adduct-d3; Deuterated PhIP-Deoxyguanosine Adduct; 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-C8-deoxyguanosine Adduct (deuterated); N-(Deoxyguanosin-8-yl)-2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-d3; C8-PhIP-dG Adduct
EINECS	Contact for details

Quality Control

This high-purity analytical standard is manufactured under strict quality control conditions. Each batch is characterized using advanced spectroscopic and chromatographic techniques, including NMR and High-Resolution Mass Spectrometry (HRMS), to confirm structure, isotopic enrichment, and purity. Certificates of Analysis (COA) detailing batch-specific data for identity, purity (by HPLC), and isotopic purity are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (NMR)	Conforms to structure
Identification (HRMS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (D3)	≥ 98 atom % D
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.