Description

Uracil-2-13C is a stable isotope-labeled analog of the fundamental pyrimidine nucleobase uracil, specifically enriched with carbon-13 at the 2-position. This precise isotopic labeling is critical for advanced analytical and metabolic research, providing a non-radioactive tracer for accurate quantification and pathway elucidation. It is an essential material for researchers and manufacturers in pharmaceutical development, clinical diagnostics, and metabolic studies requiring high-fidelity isotopic standards.

Application

• Isotope Dilution Mass Spectrometry (IDMS): Serves as an internal standard for the precise and accurate quantification of uracil and its derivatives in complex biological matrices.
• Metabolic Flux Analysis (MFA): Used as a tracer to study the dynamics of pyrimidine synthesis and salvage pathways in cell cultures, tissues, and whole organisms.
• Pharmaceutical R&D: Employed in the development and validation of bioanalytical methods for novel nucleoside-based therapeutics and in drug metabolism and pharmacokinetics (DMPK) studies.
• Clinical Diagnostics: Facilitates the accurate measurement of uracil and related metabolites in plasma or urine for diagnosing inborn errors of metabolism, such as hereditary orotic aciduria.
• Biochemical Research: A key tool for investigating RNA synthesis, turnover, and the enzymatic mechanisms of uracil-processing enzymes like dihydropyrimidine dehydrogenase (DPD).
• Nutrient Tracer Studies: Applied in nutritional science to trace the incorporation of pyrimidines into nucleic acids and understand nucleotide metabolism.

Basic Information

Product Name	Uracil-2-13C
CAS No.	35803-45-3
Molecular Formula	C313CH4N2O2
Molecular Weight	113.07 g/mol
Synonyms	2,4(1H,3H)-Pyrimidinedione-2-13C; [2-13C]Uracil; 2-13C-Uracil; 13C-Labeled Uracil; Uracil (2-13C); Isotopic Uracil; 2,4-Dihydroxypyrimidine-2-13C; NSC 27420-2-13C
EINECS	Contact for details

Quality Control

Every batch of Uracil-2-13C is produced and tested under strict quality management protocols to ensure the highest standards of isotopic and chemical purity, which are paramount for research integrity. Our products undergo rigorous quality testing, including NMR and MS for isotopic enrichment verification and HPLC for chemical purity. Certificates of Analysis (COA) detailing batch-specific results for assay, isotopic purity, and identified impurities are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which could affect stability and analytical performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Purity (NMR/MS)	≥ 99 atom % 13C
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.