Description

N,N'-Didansyl-L-Cystine CAS NO 32442-99-2 is a high-purity, dansyl-labeled derivative of the amino acid cystine, designed for advanced analytical and research applications. This compound serves as a critical reagent in fluorescence-based detection and quantification methods, offering exceptional sensitivity and specificity. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors for protein analysis, biomarker studies, and advanced chromatographic applications.

Application

• Fluorescent Labeling Agent: Used as a derivatization reagent for the pre-column or post-column labeling of peptides, proteins, and thiol-containing compounds in High-Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (CE) to enhance detection sensitivity.
• Biochemical Research: Employed in studying protein structure, function, and folding, particularly in disulfide bond mapping and thiol/disulfide exchange reactions due to its specific reactivity with cystine residues.
• Pharmaceutical Analysis: Applied in the development and validation of analytical methods for the quantification of cysteine/cystine in drug substances, formulations, and biological samples.
• Diagnostic Assay Development: Utilized as a key component in developing fluorescent probes and assay kits for detecting oxidative stress markers and metabolic disorders related to glutathione metabolism.
• Proteomics and Metabolomics: Serves as a standard or reference compound in mass spectrometry and liquid chromatography-mass spectrometry (LC-MS) workflows for accurate identification and quantification.

Basic Information

Item	Detail
Product Name	N,N'-Didansyl-L-Cystine
CAS No.	32442-99-2
Molecular Formula	C30H32N4O6S2
Molecular Weight	608.73 g/mol
Synonyms	L-Cystine, N,N'-didansyl-; Didansylcystine; N,N'-Bis(5-dimethylaminonaphthalene-1-sulfonyl)-L-cystine; Dansyl cystine; DNS-Cystine; 5-(Dimethylamino)naphthalene-1-sulfonyl cystine derivative; Fluorescent cystine derivative
EINECS	Contact for details

Quality Control

Our N,N'-Didansyl-L-Cystine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, identity confirmation by IR and NMR spectroscopy, and precise determination of residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research or production specifications. Our quality commitment supports applications requiring high batch-to-batch consistency and reliable performance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). For long-term storage, consider storing desiccated at -20°C. Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.