Description

Uridine, [5-3H] is a tritium-labeled nucleoside analog, specifically radiolabeled at the 5-position of the uracil ring. This compound is essential for metabolic pathway tracing and nucleic acid research, providing a critical tool for studying RNA synthesis, cell proliferation, and nucleoside transport mechanisms. It is primarily utilized by research scientists and pharmaceutical developers in the fields of biochemistry, molecular biology, and radiopharmaceutical development.

Application

• Biochemical Research: As a tracer for studying de novo and salvage pathways of pyrimidine nucleotide biosynthesis.
• Molecular Biology: Radiolabeling of RNA in cell culture to investigate transcription rates, RNA turnover, and gene expression.
• Cell Proliferation Assays: Used to measure DNA/RNA synthesis rates as an indicator of cellular growth and division.
• Pharmacokinetic Studies: Tracing the absorption, distribution, metabolism, and excretion (ADME) of nucleoside-based drugs.
• Enzymology: Substrate for enzymes such as uridine kinase and other nucleoside-metabolizing enzymes.
• Autoradiography & Imaging: Enables precise localization of RNA synthesis in tissues and cells.

Basic Information

Product Name	Uridine, [5-3H]
CAS No.	3705-45-1
Molecular Formula	C9H113HN2O6 *
Molecular Weight	Contact for details
Synonyms	5-Tritiouracil Riboside; [5-3H]Uridine; Tritiated Uridine; Uridine-5-3H; 1-β-D-Ribofuranosyl-[5-3H]uracil; Uridine (tritium labeled); 5-Tritio-1-β-D-ribofuranosyluracil
EINECS	Contact for details

* Formula represents the unlabeled base structure; exact molecular weight varies with specific activity.

Quality Control

Our Uridine, [5-3H] is produced and handled under stringent quality protocols to ensure radiochemical purity and specific activity consistency. Each batch is analyzed via HPLC and liquid scintillation counting. A comprehensive Certificate of Analysis (COA) detailing specific activity, radiochemical purity, chemical purity, and solvent composition is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the solution at or below -20°C. Avoid repeated freeze-thaw cycles. All handling must comply with local regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Specific Activity	10-40 Ci/mmol (Contact for specific value)
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 95%
Concentration	1 mCi/mL in ethanol:water (7:3)
Identification (TLC/Co-chromatography)	Conforms to standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.