Description

L-Isoleucine (1-13C) is a stable isotope-labeled analog of the essential branched-chain amino acid L-Isoleucine, where the carbon at the 1-position is enriched with the non-radioactive carbon-13 isotope. This compound is a critical tool for advanced research, enabling precise metabolic pathway tracing and quantitative analysis in complex biological systems. It is primarily utilized by scientists and manufacturers in the pharmaceutical, biotechnology, and nutritional research sectors for applications in drug development, metabolic studies, and stable isotope labeling of proteins and peptides.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to study and quantify intracellular metabolic pathways in cell cultures, tissues, and whole organisms.
• Proteomics & Protein Labeling: Essential for Stable Isotope Labeling by Amino acids in Cell culture (SILAC) and other mass spectrometry-based techniques for accurate protein quantification and identification.
• Pharmaceutical Research & Development: Employed in the development and pharmacokinetic/pharmacodynamic (PK/PD) studies of new therapeutic agents, particularly those targeting amino acid metabolism.
• Nutritional Science: Applied in research to understand protein metabolism, amino acid requirements, and the metabolic fate of dietary components in humans and animals.
• Biochemical Research: Serves as a substrate or precursor in enzymatic studies and the biosynthesis of labeled complex molecules.
• Diagnostic Agent Development: Potential use in the formulation of advanced diagnostic probes for medical imaging techniques like magnetic resonance spectroscopy (MRS).

Basic Information

Product Name	L-Isoleucine (1-13C)
CAS No.	2687960-40-1
Molecular Formula	C613CH13NO2
Molecular Weight	132.18 g/mol (based on 13C at C1)
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic-1-13C acid; L-Isoleucine-1-13C; (2S,3S)-2-Azaniumyl-3-methylpentanoate-1-13C; ILE-1-13C; (S,S)-Isoleucine-1-13C; Isotopically Labeled L-Isoleucine; 13C1-L-Isoleucine
EINECS	Contact for details

Quality Control

Our L-Isoleucine (1-13C) is manufactured under strict quality systems to ensure the highest standards of isotopic and chemical purity. Each batch is subjected to rigorous analytical testing, including NMR, HPLC, and Mass Spectrometry, to confirm identity, isotopic enrichment (typically >99 atom % 13C), and chemical purity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive); keep the container tightly sealed in a dry environment.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
13C Isotopic Enrichment	≥ 99 atom %
Assay (HPLC)	≥ 98.0%
Optical Rotation	[α]D20: +39.5° to +41.5° (c=4 in 6N HCl)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.