Description

Dl-Histidine-A-15N is a stable isotope-labeled analog of the amino acid histidine, where the alpha nitrogen atom is enriched with Nitrogen-15. This compound is essential for advanced research and development in life sciences, providing a critical tool for precise metabolic and proteomic studies. It is primarily utilized by pharmaceutical researchers, academic institutions, and diagnostic developers requiring high-fidelity isotopic tracers for mass spectrometry, NMR spectroscopy, and pharmacokinetic research.

Application

• As an internal standard for the accurate quantification of histidine in biological samples via LC-MS/MS and GC-MS.
• A key precursor in the synthesis of Nitrogen-15 labeled peptides and proteins for structural biology and NMR-based research.
• Use in metabolic flux analysis to trace and model amino acid metabolism in cell cultures and animal studies.
• Critical component in stable isotope labeling by amino acids in cell culture (SILAC) workflows for quantitative proteomics.
• Research into histidine metabolism disorders and related biochemical pathways.
• Development and calibration of in-vitro diagnostic (IVD) assays and clinical mass spectrometry methods.

Basic Information

Product Name	Dl-Histidine-A-15N
CAS No.	287484-37-1
Molecular Formula	C₆H₉¹⁵N₃O₂
Molecular Weight	158.14 g/mol (for ¹⁵N labeled form)
Synonyms	DL-Histidine (α-15N); Histidine-α-15N; (RS)-Histidine-α-15N; 2-Amino-3-(1H-imidazol-4-yl)propanoic acid-α-15N; (±)-Histidine-α-15N; L-Histidine-α-15N (isomer reference); Histidine (A-15N); ¹⁵N-α-L-Histidine
EINECS	Contact for details

Quality Control

Our Dl-Histidine-A-15N is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % ¹⁵N), chemical purity, and specific analytical results from techniques such as NMR and HPLC. We support compliance with relevant GMP and ISO guidelines for research chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % ¹⁵N)	≥ 98%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.