Description

L-Leucine-2-13C,15N is a stable isotope-labeled analog of the essential amino acid L-leucine, specifically enriched with carbon-13 at the 2-position and nitrogen-15. This compound is a critical tool for advanced metabolic research, enabling precise tracking and quantification of amino acid metabolism, protein synthesis, and nutrient utilization in complex biological systems. It is primarily utilized by researchers and manufacturers in the pharmaceutical, nutritional science, and biotechnology sectors who require high-fidelity tracers for mass spectrometry (MS) and nuclear magnetic resonance (NMR) studies.

Application

• Metabolic Pathway Tracing: Used as a stable isotope tracer in in vivo and in vitro studies to investigate leucine metabolism, protein turnover, and branched-chain amino acid (BCAA) catabolism.
• Quantitative Proteomics & Biomarker Discovery: Essential for SILAC (Stable Isotope Labeling by Amino acids in Cell culture) and other mass spectrometry-based techniques for accurate protein quantification and identification.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Employed in drug development to study the absorption, distribution, metabolism, and excretion (ADME) of new therapeutic compounds that interact with amino acid pathways.
• Nutritional Research: Applied in clinical and sports nutrition studies to assess muscle protein synthesis rates, metabolic responses to feeding, and the efficacy of nutritional supplements.
• Diagnostic Agent Development: Serves as a precursor for synthesizing labeled compounds used in advanced medical diagnostics, including positron emission tomography (PET) tracers.
• Quality Control Reference Standard: Acts as an internal standard in analytical laboratories for the precise quantification of L-leucine in complex matrices like biological fluids, food products, and pharmaceuticals.

Basic Information

Product Name	L-Leucine-2-13C,15N
CAS No.	285977-88-0
Molecular Formula	C513CH1315NO2
Molecular Weight	134.15 g/mol (for 13C1, 15N1 labeled form)
Synonyms	(2S)-2-Azanyl-4-methyl(13C1,15N)pentanoic acid; L-(2-13C,15N)Leucine; (S)-2-Amino-4-methyl(13C1,15N)pentanoic acid; Leucine-2-13C,15N; [2-13C,15N]-L-Leucine; Stable Isotope Labeled L-Leucine; 13C,15N-L-Leu
EINECS	Contact for details

Quality Control

Our L-Leucine-2-13C,15N is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is analyzed to confirm isotopic enrichment (Atom % 13C and 15N), chemical purity, and optical purity. Certificates of Analysis (COA) detailing lot-specific results from NMR, HPLC, and MS are provided and available upon request. Production can adhere to cGMP guidelines for advanced pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation due to moisture absorption. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure and labeling pattern
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 13C)	≥ 99%
Isotopic Enrichment (Atom % 15N)	≥ 98%
Optical Rotation	[α]D20: +14.5° to +16.5° (c=2 in 6N HCl)
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.