Description

Tris-Succinimidyl-1,3,5-Benzenetricarboxylate CAS NO 268539-19-1 is a high-purity, trifunctional N-hydroxysuccinimide (NHS) ester crosslinker. This compound is critically important for creating stable amide bonds with primary amines, enabling the efficient conjugation of biomolecules in aqueous buffers. It is an essential reagent for researchers and manufacturers in bioconjugation, proteomics, and the development of diagnostic assays and therapeutic agents. Its symmetrical structure allows for controlled multi-point attachment, making it ideal for constructing complex molecular architectures.

Application

• Bioconjugation and Crosslinking: Primary reagent for covalently linking proteins, peptides, antibodies, and other amine-containing biomolecules to surfaces, particles, or other molecules.
• Proteomics Research: Used in mass spectrometry workflows for crosslinking mass spectrometry (XL-MS) to study protein-protein interactions and 3D structures.
• Diagnostic Assay Development: Key component in the manufacture of enzyme-linked immunosorbent assays (ELISAs), lateral flow assays, and other immunoassay platforms by conjugating detection enzymes to antibodies.
• Biomaterial and Hydrogel Fabrication: Serves as a crosslinking agent for amine-functionalized polymers in the creation of controlled-release systems, tissue engineering scaffolds, and smart materials.
• Antibody-Drug Conjugate (ADC) Research: Employed in early-stage research for exploring linker chemistry and conjugation strategies for targeted cancer therapies.
• Nanoparticle Functionalization: Used to attach targeting ligands (e.g., peptides, antibodies) to the surface of nanoparticles for drug delivery and imaging applications.
• Immobilization Chemistry: Facilitates the covalent attachment of biomolecules to solid supports such as beads, chips, and sensor surfaces for chromatography, purification, and biosensing.

Basic Information

Product Name	Tris-Succinimidyl-1,3,5-Benzenetricarboxylate
CAS No.	268539-19-1
Molecular Formula	C21H15N3O12
Molecular Weight	501.36 g/mol
Synonyms	1,3,5-Benzenetricarboxylic Acid Tris(N-succinimidyl) Ester; Tris(N-succinimidyl) 1,3,5-Benzenetricarboxylate; TSBTC; Tris(succinimidyl) Benzenetricarboxylate; NSC 339458; 1,3,5-Tris(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzene; Benzoic Acid, 1,3,5-Tricarboxylic Acid, Tris(2,5-dioxo-1-pyrrolidinyl) Ester
EINECS	Contact for details

Quality Control

Our Tris-Succinimidyl-1,3,5-Benzenetricarboxylate is manufactured under strict quality protocols to ensure batch-to-batch consistency and high reactivity. Each lot is characterized to confirm identity and purity, meeting the stringent requirements for life science research and development. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMF, DMSO; partially soluble in acetonitrile
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by amine coupling test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.