Description

L-Phenylalanine-D5 Methyl Ester Hydrochloride is a stable isotope-labeled derivative of the essential amino acid L-phenylalanine, where five hydrogen atoms are replaced by deuterium. This high-purity compound is critical for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical measurements. It is primarily needed by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in advanced metabolic studies, pharmacokinetic research, and proteomics.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a crucial internal standard for the precise quantification of L-phenylalanine and related metabolites in complex biological matrices like plasma, serum, and tissue homogenates.
• Pharmacokinetic and Bioavailability Studies: Enables accurate tracking and measurement of drug absorption, distribution, metabolism, and excretion (ADME) for pharmaceuticals targeting amino acid pathways.
• Metabolomics and Biomarker Discovery: Used in high-throughput screening to identify and quantify disease-specific metabolic signatures related to phenylalanine metabolism, such as in phenylketonuria (PKU) research.
• Proteomics and Peptide Analysis: Employed in stable isotope labeling by amino acids in cell culture (SILAC) and other techniques for relative and absolute protein quantification.
• Quality Control in Pharmaceutical Manufacturing: Acts as a reference standard to validate analytical methods and ensure the purity and consistency of amino acid-based active pharmaceutical ingredients (APIs).
• Nutritional and Clinical Research: Facilitates studies on amino acid turnover, protein synthesis rates, and metabolic disorders in human and animal models.

Basic Information

Product Name	L-Phenylalanine-D5 Methyl Ester Hydrochloride
CAS No.	213547-79-6
Molecular Formula	C10H8D5ClNO2
Molecular Weight	215.70 g/mol (for unlabeled form; exact mass varies with isotopic enrichment)
Synonyms	(S)-2-Amino-3-(2,3,4,5,6-pentadeuterophenyl)propanoic acid methyl ester hydrochloride; Methyl L-phenylalanine-2,3,4,5,6-D5 hydrochloride; L-Phe(D5)-OMe·HCl; Phenylalanine-d5 methyl ester HCl; Deuterated L-Phenylalanine methyl ester hydrochloride; Methyl (2S)-2-amino-3-(pentadeuterophenyl)propanoate hydrochloride; L-Phenylalanine (phenyl-d5) methyl ester hydrochloride
EINECS	Contact for details

Quality Control

Our L-Phenylalanine-D5 Methyl Ester Hydrochloride is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including NMR, HPLC, and mass spectrometry, to confirm chemical identity, isotopic purity (typically >98 atom % D), and chemical purity (typically >95%). Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere after each use to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Isotopic Purity (NMR/MS)	≥ 98 atom % D
Chemical Purity	≥ 95%
Specific Rotation	Contact for details
Residual Solvents (GC)	Meets ICH guidelines
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.