Description

L-Histidine Hydrochloride Hydrate (<5% D) (13C6, 97-99%) is a stable isotope-labeled derivative of the essential amino acid L-Histidine, specifically enriched with carbon-13 (13C6) and with a defined low level of deuterium (D) incorporation. This high-purity, isotopically enriched compound is critical for applications requiring precise quantification and metabolic pathway tracing, where it serves as an internal standard to ensure analytical accuracy and reliability. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors for advanced mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, and clinical diagnostics.

Application

• Quantitative Mass Spectrometry (MS) & LC-MS/MS: Serves as a high-purity internal standard for the accurate quantification of L-Histidine and related metabolites in complex biological matrices such as plasma, urine, and tissue samples.
• Metabolic Flux Analysis & Tracer Studies: Used in 13C metabolic flux analysis to study histidine metabolism, protein synthesis rates, and related biochemical pathways in cell cultures, tissues, or whole organisms.
• Nuclear Magnetic Resonance (NMR) Spectroscopy: Provides a non-radioactive, stable isotope label for structural elucidation, protein dynamics studies, and investigating enzyme mechanisms involving histidine residues.
• Clinical Research & Diagnostics: Essential for developing and validating high-sensitivity diagnostic assays for inborn errors of metabolism, nutritional status assessment, and biomarker discovery related to histidine metabolism.
• Pharmaceutical R&D: Employed in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track the absorption, distribution, metabolism, and excretion (ADME) of drug candidates containing or affecting histidine pathways.
• Nutritional Science Research: Used in stable isotope feeding studies to investigate protein turnover, amino acid requirements, and the metabolic fate of dietary histidine.

Basic Information

Product Name	L-Histidine Hydrochloride Hydrate (<5% D) (13C6, 97-99%)
CAS No.	202468-43-7
Molecular Formula	C613C6H9D<0.3N3O2 • HCl • xH2O
Molecular Weight	~221.6 (anhydrous free base, 13C6)
Synonyms	(2S)-2-Amino-3-(1H-imidazol-4-yl)propanoic-13C6 acid hydrochloride hydrate (D<5%); L-Histidine-13C6 hydrochloride hydrate; Histidine-13C6 HCl hydrate; (S)-Histidine-13C6 monohydrochloride hydrate; 13C6-L-Histidine HCl hydrate; Stable Isotope Labeled L-Histidine HCl; Isotopic Internal Standard L-Histidine HCl
EINECS	Contact for details

Quality Control

Every batch of L-Histidine Hydrochloride Hydrate (<5% D) (13C6, 97-99%) is manufactured and analyzed under strict quality systems. The product undergoes comprehensive testing to ensure it meets the stringent requirements for isotopic purity, chemical purity, and isotopic enrichment essential for analytical and research applications. Certificates of Analysis (COA) are provided, detailing key parameters such as isotopic purity (Atom % 13C), chemical purity by HPLC, chiral purity, residual solvent content, and water content (KF). Our quality commitment supports compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 97.0%
Isotopic Purity (Atom % 13C)	97 - 99%
Deuterium Content (D)	< 5%
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.