Description

L-Isoleucine (U-13C6,15N) is a uniformly carbon-13 and nitrogen-15 labeled isotopologue of the essential branched-chain amino acid L-Isoleucine, with CAS NO 202468-35-7. This high-purity stable isotope-labeled compound is critical for advanced research requiring precise metabolic tracing and quantification. It is primarily utilized by scientists and manufacturers in pharmaceutical development, metabolic research, and clinical diagnostics for applications such as mass spectrometry-based proteomics and metabolic flux analysis.

Application

• Metabolic Pathway Tracing: Used as an internal standard in mass spectrometry (MS) and nuclear magnetic resonance (NMR) studies to track and quantify metabolic fluxes in cell cultures, tissues, and whole organisms.
• Quantitative Proteomics: Serves as a crucial component in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for accurate protein quantification and biomarker discovery.
• Pharmaceutical Research: Employed in the development and pharmacokinetic studies of new drug candidates, particularly those targeting amino acid metabolism.
• Clinical Diagnostics: Used as a reference standard in assays for inborn errors of metabolism, such as Maple Syrup Urine Disease (MSUD), to ensure accurate diagnosis.
• Nutritional Science Research: Applied in studies investigating protein synthesis, muscle metabolism, and the metabolic fate of dietary amino acids.
• Biochemical Reagent: Acts as a high-purity labeled substrate for enzymatic assays and studies of enzyme kinetics and mechanisms.

Basic Information

Product Name	L-Isoleucine (U-13C6,15N)
CAS No.	202468-35-7
Molecular Formula	¹³C₆H₁₃¹⁵NO₂
Molecular Weight	136.13 (for ¹³C₆H₁₃¹⁵NO₂)
Synonyms	(2S,3S)-2-Amino-3-methylpentanoic acid (U-13C6,15N); (2S,3S)-2-Azaniumyl-3-methylpentanoate (U-13C6,15N); L-2-Amino-3-methylvaleric acid (U-13C6,15N); (S)-Isoleucine (U-13C6,15N); Iso-Leucine (U-13C6,15N); Ile (U-13C6,15N); Uniformly Labeled L-Isoleucine-13C6,15N; U-13C6,15N-L-Isoleucine
EINECS	Contact for details

Quality Control

Our L-Isoleucine (U-13C6,15N) is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research applications. Each batch is analyzed to confirm identity, isotopic enrichment (Atom % 13C and 15N), chemical purity, and the absence of critical impurities. Certificates of Analysis (COA) detailing specific lot data, including NMR and MS confirmation, are provided to guarantee traceability and compliance with your research protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation due to moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 13C)	≥ 99%
Isotopic Enrichment (Atom % 15N)	≥ 98%
Optical Rotation	[α]20/D: +39.5° to +41.5° (c=4 in 6N HCl)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.