Description

5-Methyl-[1'-13C]Uridine is a stable isotope-labeled nucleoside analog, specifically labeled with carbon-13 at the 1' position of the ribose sugar. This precise isotopic labeling is critical for advanced research applications requiring high sensitivity and specificity in metabolic and pharmacokinetic studies. It is an essential tool for scientists in pharmaceutical R&D, academic research, and contract research organizations (CROs) focused on nucleoside metabolism, RNA research, and the development of antiviral or anticancer therapeutics.

Application

• Isotope Tracer Studies: Used as a metabolic tracer to investigate the biosynthesis, turnover, and catabolic pathways of RNA and modified nucleosides in biological systems.
• Mass Spectrometry (MS) Internal Standard: Serves as a critical internal standard for the accurate quantification of 5-methyluridine and related metabolites via LC-MS/MS, enhancing analytical precision and reliability.
• Pharmaceutical R&D: Employed in drug metabolism and pharmacokinetics (DMPK) studies for labeled drug candidates, aiding in absorption, distribution, metabolism, and excretion (ADME) profiling.
• Epigenetics & RNA Modification Research: A vital reagent for studying the role and dynamics of RNA modifications, particularly in the field of epitranscriptomics.
• Diagnostic Assay Development: Utilized in the development of clinical diagnostic assays for detecting specific metabolic disorders or disease biomarkers.

Basic Information

Product Name	5-Methyl-[1'-13C]Uridine
CAS No.	201996-60-3
Molecular Formula	C10H1413CN2O6
Molecular Weight	260.22 g/mol (for 13C labeled form)
Synonyms	1'-13C-5-Methyluridine; [1'-13C]-5-Methyluridine; 5-Methyl-1'-13C-uridine; m5U-[1'-13C]; Ribothymidine-1'-13C; 1-(β-D-Ribofuranosyl)-5-methyl[1'-13C]uracil; 5-Methyl-1-β-D-ribofuranosyl[1'-13C]uracil
EINECS	Contact for details

Quality Control

Every batch of 5-Methyl-[1'-13C]Uridine is manufactured and analyzed under strict quality control protocols to ensure the highest isotopic and chemical purity for research applications. Our standard quality commitment includes comprehensive analysis via NMR (for isotopic enrichment verification) and HPLC. Certificates of Analysis (COA) are provided with each shipment, detailing key parameters such as chemical purity, isotopic purity (atom % 13C), and specific analytical data. We adhere to cGMP principles for the production of critical research materials.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This compound is hygroscopic (moisture-sensitive); desiccants are recommended for long-term storage. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (atom % 13C)	≥ 99%
Isotopic Enrichment (NMR/MS)	≥ 99% at 1' position
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.