Description

Cytosine (2-13C, 99%) is a stable isotope-labeled analog of the fundamental pyrimidine nucleobase cytosine, specifically enriched with carbon-13 at the 2-position. This high isotopic purity (99%) is critical for ensuring accuracy and reliability in advanced analytical and metabolic research. It is an essential reagent for scientists in pharmaceutical development, biochemistry, and metabolomics who require precise molecular tracers. The compound is primarily used for NMR spectroscopy, mass spectrometry, and tracing studies in drug metabolism and biochemical pathway analysis.

Application

• NMR Spectroscopy: Serves as an internal standard or probe for structural elucidation and dynamic studies of nucleic acids and related compounds.
• Mass Spectrometry (MS): Used as a labeled standard for the quantitative analysis of cytosine and its derivatives in complex biological matrices.
• Drug Metabolism & Pharmacokinetics (DMPK): Enables precise tracing of the metabolic fate of nucleoside-based pharmaceuticals.
• Biochemical Pathway Analysis: Facilitates the study of nucleotide synthesis, salvage pathways, and cellular metabolism in research.
• Proteomics & Metabolomics: Acts as a key component in isotope-coded affinity tag (ICAT) or similar labeling strategies for quantitative profiling.
• Stable Isotope Labeling in Cell Culture (SILAC) for Nucleic Acids: Used in advanced cell culture studies to track nucleic acid turnover and synthesis.

Basic Information

Product Name	Cytosine (2-13C, 99%)
CAS No.	201417-11-0
Molecular Formula	C413CH6N3O
Molecular Weight	112.09 g/mol (for 13C1 isotopologue)
Synonyms	4-Amino-2(1H)-pyrimidinone-2-13C; 2-13C-Cytosine; 6-Amino-2(1H)-pyrimidinone-2-13C; Cytosine-2-13C; [2-13C]Cytosine; 4-Aminopyrimidin-2(1H)-one-2-13C; 2-13C Labeled Cytosine; Isotope-labeled Cytosine
EINECS	Contact for details

Quality Control

Every batch of Cytosine (2-13C, 99%) is produced and analyzed under strict quality management protocols to ensure the highest isotopic and chemical purity. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, providing detailed data for parameters including isotopic enrichment (by NMR or MS), chemical purity (by HPLC), and identity confirmation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Enrichment (13C at C-2)	≥ 99 atom % 13C
Chemical Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.