Description

Orotic Acid, [5-3H] is a tritium-labeled derivative of orotic acid, a key intermediate in pyrimidine biosynthesis. This labeled compound is essential for precise metabolic tracing and pharmacokinetic studies in biochemical and pharmaceutical research. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors for advanced analytical applications.

Application

• Biochemical Research: As a radiolabeled tracer for studying the de novo biosynthesis pathway of pyrimidine nucleotides.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in ADME (Absorption, Distribution, Metabolism, Excretion) studies to track the metabolic fate of drug candidates.
• Enzyme Assay Development: Serving as a substrate in assays for enzymes like dihydroorotate dehydrogenase (DHODH).
• Nutritional Science: Investigating the role of orotic acid in lipid metabolism and liver function.
• Diagnostic Agent Development: Potential use in the research and development of novel diagnostic probes.
• Quality Control (QC): Acting as an internal standard or reference material in analytical methods such as HPLC or LC-MS for pharmaceutical QC.

Basic Information

Product Name	Orotic Acid, [5-3H]
CAS No.	24465-39-2
Molecular Formula	C5H4N2O4 (³H)
Molecular Weight	~156.10 g/mol (³H labeled)
Synonyms	6-Carboxyuracil, [5-³H]; Vitamin B13 (labeled); 1,2,3,6-Tetrahydro-2,6-dioxo-4-pyrimidinecarboxylic acid, [5-³H]; L-4-Carboxyuracil, [5-³H]; Wheeler-LaMer Acid (labeled); Animal Galactose Factor (labeled); Orotate, [5-³H]; [5-³H]-Orotic Acid
EINECS	Contact for details

Quality Control

Our Orotic Acid, [5-3H] is produced and handled under stringent quality systems. Each batch undergoes comprehensive analytical testing, including radiochemical purity and specific activity determination by HPLC with radiometric detection, to ensure it meets the exacting standards required for research use. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a controlled environment to minimize exposure to atmospheric moisture. For long-term storage, consider conditions recommended for radiolabeled compounds.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Chemical Purity (HPLC)	≥ 98%
Solvent (for solutions)	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.