Description

5-Fluorouracil, [6-3H]- is a tritium-labeled derivative of the widely used antimetabolite and chemotherapeutic agent, 5-fluorouracil. This radiolabeled compound is an essential tool for advanced pharmacokinetic, metabolic, and biochemical research, enabling precise tracking and quantification in biological systems. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and contract research organizations (CROs) focused on oncology, drug metabolism, and molecular biology studies.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Tracing the absorption, distribution, metabolism, and excretion (ADME) pathways of 5-fluorouracil in preclinical models.
• Biochemical Mechanism Research: Investigating the precise mechanisms of action, including thymidylate synthase inhibition and incorporation into RNA/DNA.
• Autoradiography and Imaging: Used in tissue distribution studies and receptor binding assays to visualize drug localization at the cellular level.
• Metabolic Pathway Analysis: Elucidating catabolic and anabolic pathways of fluoropyrimidines in both target and non-target tissues.
• Quality Control for Radiolabeled Formulations: Serving as a reference standard in the development and validation of other radiolabeled drug products.
• Environmental Fate Studies: Researching the environmental persistence and breakdown of fluorouracil compounds (specialized applications).

Basic Information

Product Name	5-Fluorouracil, [6-3H]-
CAS No.	23935-92-4
Molecular Formula	C4H2FN2O2 (3H)
Molecular Weight	130.08 g/mol (unlabeled)
Synonyms	5-FU-6-3H; [6-3H]-5-Fluorouracil; 5-Fluoro-2,4(1H,3H)-pyrimidinedione-6-3H; 5-FU-tritiated; Tritiated 5-Fluorouracil; 5-Fluorouracil (tritium labeled); NSC-19893 (tritiated)
EINECS	Contact for details

Quality Control

Every batch of 5-Fluorouracil, [6-3H]- is manufactured and analyzed under strict quality systems. We provide comprehensive analytical data to ensure product integrity for critical research applications. Certificates of Analysis (COA) are supplied with each shipment, detailing parameters such as radiochemical purity, specific activity, chemical purity, and solvent composition. Our quality commitment aligns with the standards expected in pharmaceutical and high-level academic research.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as specified on the label. For long-term storage, keep in a dedicated, well-ventilated freezer designated for radioactive materials. Users must adhere to all local and national regulations for the handling and storage of radioactive compounds.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC/Co-injection)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	10-30 Ci/mmol (Contact for exact value)
Chemical Purity (HPLC, UV)	≥ 98%
Solvent	Ethanol/Water or as specified
Concentration	1 mCi/mL (Typical, custom available)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.