Description

L-Histidine-2,5-3H CAS NO 22835-06-9 is a tritium-labeled derivative of the essential amino acid L-Histidine, specifically labeled at the 2 and 5 positions of the imidazole ring. This radiolabeled compound is a critical tool for advanced biochemical and pharmacological research, enabling precise metabolic and pharmacokinetic studies. It is primarily utilized by research scientists and pharmaceutical developers in tracer studies, receptor binding assays, and drug metabolism investigations.

Application

• Biochemical Tracer Studies: Used to track the metabolic fate, absorption, and distribution of histidine and histidine-containing compounds in biological systems.
• Pharmacokinetic & ADME Research: Essential for studying the Absorption, Distribution, Metabolism, and Excretion (ADME) profiles of new drug candidates in preclinical development.
• Receptor Binding & Enzyme Assays: Serves as a high-specific-activity ligand in competitive binding studies for histamine receptors and research on histidine-decarboxylating enzymes.
• Protein & Peptide Research: Employed in the radiolabeling and turnover studies of proteins and peptides that incorporate histidine.
• Nutritional & Metabolic Research: Facilitates investigations into histidine requirements, transport mechanisms, and its role in various metabolic pathways.
• Autoradiography & Imaging: Provides the radioactive source for detailed autoradiographic imaging in tissue sections for localization studies.

Basic Information

Item	Detail
Product Name	L-Histidine-2,5-3H
CAS No.	22835-06-9
Molecular Formula	C6H93H2N3O2
Molecular Weight	~158.16 g/mol (varies with specific activity)
Synonyms	L-Histidine (2,5-3H); (2S)-2-Amino-3-(1H-imidazol-4-yl-2,5-3H)propanoic acid; Histidine-2,5-t; Tritiated L-Histidine; [2,5-3H]-L-Histidine; L-Histidine-2,5-t2; Histidine (L-) tritiated
EINECS	Contact for details

Quality Control

Every batch of L-Histidine-2,5-3H is manufactured and analyzed under strict quality systems to ensure the highest standards of radiochemical purity and specific activity. Our products undergo rigorous quality testing, including HPLC analysis for radiochemical purity and identity confirmation. Certificates of Analysis (COA) detailing specific activity, purity, solvent composition, and analytical methods are provided with each shipment to ensure full traceability and compliance with research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C. This product is hygroscopic (moisture-sensitive). For long-term storage, solutions are recommended to be kept in appropriate shielded containers in a dedicated freezer. Always follow local radiation safety regulations for handling and storage.

Specification

Item	Specification
Appearance	Colorless to pale yellow solution (in specified solvent)
Identification (HPLC)	Conforms to reference standard
Radiochemical Purity (HPLC)	≥ 95%
Specific Activity	Customizable (Typical range: 10-100 Ci/mmol)
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol/Water or as specified
Concentration	1 mCi/mL (or as specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.