Description

L-Alanine-1-13C CAS NO 21764-56-7 is a stable isotope-labeled analog of the essential amino acid L-Alanine, where the carbon at the 1-position is enriched with the 13C isotope. This compound is a critical tool in advanced research and analytical chemistry, enabling precise metabolic pathway tracing, quantitative proteomics, and the development of internal standards for mass spectrometry. It is primarily required by pharmaceutical R&D laboratories, academic research institutions, and diagnostic manufacturers engaged in isotope dilution mass spectrometry (IDMS), nuclear magnetic resonance (NMR) spectroscopy, and metabolic flux analysis.

Application

• Internal Standard for Quantitative Mass Spectrometry (MS): Serves as a critical reference compound in LC-MS/MS and GC-MS for the accurate quantification of endogenous L-alanine in complex biological matrices like plasma, urine, and tissue samples.
• Metabolic Pathway Tracing (Fluxomics): Used in 13C metabolic flux analysis (13C-MFA) to study central carbon metabolism in cells, bacteria, and plants, providing insights into metabolic network activity and regulation.
• Proteomics and Peptide Synthesis: Incorporated into isotopically labeled peptides and proteins for use in absolute quantification (AQUA) strategies and protein turnover studies.
• NMR Spectroscopy Studies: Acts as a site-specific probe in Nuclear Magnetic Resonance spectroscopy to investigate protein structure, dynamics, and ligand interactions without spectral overlap from natural abundance signals.
• Clinical Diagnostics & Biomarker Research: Enables the development of highly accurate clinical assays for diagnosing inborn errors of metabolism related to alanine metabolism.
• Pharmaceutical Drug Metabolism & Pharmacokinetics (DMPK): Utilized to trace the metabolic fate of drug candidates containing alanine moieties or to study amino acid transporter functions.

Basic Information

Product Name	L-Alanine-1-13C
CAS No.	21764-56-7
Molecular Formula	C313CH7NO2
Molecular Weight	90.08 g/mol (for 13C1 isotopologue)
Synonyms	(2S)-2-Aminopropanoic-1-13C acid; L-α-Alanine-1-13C; (S)-Alanine-1-13C; Alanine-1-13C; L-Alanine (1-13C); 13C1-L-Alanine; 1-13C-L-Alanine; Isotope-labeled L-Alanine
EINECS	Contact for details

Quality Control

Our L-Alanine-1-13C is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing critical parameters including isotopic enrichment (Atom % 13C), chemical purity (by HPLC), chiral purity, and residual solvent content. We adhere to relevant standards for high-purity biochemicals, and custom specifications for specific research or diagnostic kit applications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. This material is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to prevent absorption of moisture, which may affect stability and accurate weighing.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Isotopic Enrichment (Atom % 13C)	≥ 99.0%
Optical Rotation	[α]D20: +13.5° to +15.5° (c=1 in H2O)
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.