Description

L-Glutamic-15N Acid is a stable isotope-labeled analog of the essential amino acid L-glutamic acid, where the nitrogen atom is enriched with the 15N isotope. This compound is a critical tool for advanced research, enabling precise metabolic and proteomic studies through mass spectrometry and NMR spectroscopy. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and manufacturers of diagnostic kits and labeled standards.

Application

• Metabolic Pathway Tracing: As an internal standard in quantitative mass spectrometry to study glutamine/glutamate metabolism in cell cultures and animal models.
• Proteomics Research: Used in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) for accurate protein quantification and turnover rate analysis.
• NMR Spectroscopy: Serves as a 15N-enriched probe for structural biology studies to investigate protein-ligand interactions and dynamics.
• Diagnostic Standard: A key component in the calibration and validation of LC-MS/MS systems for clinical diagnostics, particularly in inborn errors of metabolism testing.
• Nutrient Absorption Studies: Employed in nutritional science research to trace amino acid uptake and utilization in gastrointestinal models.
• Isotope Dilution Analysis: Provides high-accuracy quantification of glutamic acid in complex biological and food matrices.

Basic Information

Product Name	L-Glutamic-15N Acid
CAS No.	21160-87-2
Molecular Formula	C5H915NO4
Molecular Weight	148.13 g/mol (for 15N)
Synonyms	(2S)-2-Azanyl-15N-pentanedioic acid; L-Glutamic-15N acid; (S)-2-Amino-15N-glutaric acid; (S)-(+)-Glutamic-15N acid; 15N-L-Glutamic acid; GLU-15N; 2-Aminopentanedioic-15N acid; L-15N-Glutamate
EINECS	Contact for details

Quality Control

Our L-Glutamic-15N Acid is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity for sensitive research applications. Each batch is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98 atom % 15N), chemical purity, and specific optical rotation. We support compliance with cGMP and ISO 9001 standards for research material manufacturing.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C. The product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent absorption of water. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to 15N labeling pattern
Assay (HPLC)	≥98.0% (on anhydrous basis)
Isotopic Enrichment	≥98 atom % 15N
Optical Rotation	+31.5° to +32.5° (c=2 in 2N HCl)
Water Content (KF)	≤0.5%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.