Description

4-Aminophenyl B-D-Glucuronide is a high-purity biochemical reagent widely used as a critical substrate and reference standard in enzymatic and analytical research. Its primary value lies in its role as a key intermediate for the synthesis of chromogenic and fluorogenic substrates for β-glucuronidase, an enzyme of significant diagnostic and research importance. This compound is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require reliable, well-characterized materials for assay development, quality control, and biochemical studies.

Application

• Enzyme Substrate Synthesis: A key precursor for manufacturing chromogenic (e.g., X-Gluc) and fluorogenic substrates used to detect β-glucuronidase activity.
• Diagnostic Assay Development: Used in the research and production of diagnostic kits for bacterial detection (like E. coli) and reporter gene assays in molecular biology.
• Pharmaceutical Research: Serves as a reference standard and intermediate in drug metabolism studies, particularly for compounds metabolized via glucuronidation pathways.
• Biochemical Research: Employed as a tool compound to study enzyme kinetics, inhibition, and the specific activity of β-glucuronidase in various biological samples.
• Quality Control & Validation: Used as a certified reference material to calibrate equipment and validate analytical methods (HPLC, LC-MS) in QC laboratories.
• Plant Biotechnology: Applied in histological staining (GUS assay) to visualize gene expression and localization in genetically modified plants.

Basic Information

Product Name	4-Aminophenyl B-D-Glucuronide
CAS No.	21080-66-0
Molecular Formula	C12H15NO7
Molecular Weight	285.25 g/mol
Synonyms	4-Aminophenyl β-D-Glucopyranosiduronic Acid; p-Aminophenyl β-D-Glucuronide; 4-APG; APG; p-Aminophenyl Glucuronide; β-D-Glucopyranosiduronic Acid, 4-Aminophenyl Ester; 4-Aminophenyl-β-D-glucuronide; (2S,3S,4S,5R,6S)-3,4,5-Trihydroxy-6-(4-aminophenoxy)oxane-2-carboxylic Acid
EINECS	Contact for details

Quality Control

Our 4-Aminophenyl B-D-Glucuronide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets exacting specifications for research and production use. Certificates of Analysis (COA) detailing batch-specific results are available upon request. We support compliance with relevant industry standards for high-purity biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.