Description

5-Fluorouracil-6-14C is a carbon-14 radiolabeled analog of the established antimetabolite and chemotherapeutic agent, 5-fluorouracil. This isotopically labeled compound is an essential tool for conducting advanced pharmacokinetic, metabolic, and drug distribution studies in pharmaceutical research and development. It is primarily utilized by research scientists and analytical laboratories in the pharmaceutical, biotechnology, and contract research organization (CRO) sectors to trace the metabolic fate of the parent drug with high specificity and sensitivity.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used as a radiotracer to investigate the absorption, distribution, metabolism, and excretion (ADME) profile of 5-fluorouracil in preclinical models.
• Biomedical Research: Employed in mechanistic studies to elucidate the biochemical pathways and molecular targets of 5-fluorouracil's action, including thymidylate synthase inhibition.
• Analytical Method Development: Serves as a critical reference standard for developing and validating sensitive analytical methods, such as Liquid Chromatography-Mass Spectrometry (LC-MS) or accelerator mass spectrometry (AMS).
• Environmental Fate Studies: Applied in specialized research to track the environmental persistence and breakdown of fluorouracil compounds.
• Radioligand Binding Assays: Can be used in research to study drug-receptor interactions where the radiolabel provides a detectable signal.

Basic Information

Product Name	5-Fluorouracil-6-14C
CAS No.	2794-13-0
Molecular Formula	C4H3FN2O2 (with 14C at the 6-position)
Molecular Weight	~130.08 g/mol (exact mass depends on 14C enrichment)
Synonyms	5-FU-6-14C; 5-Fluoro-2,4(1H,3H)-pyrimidinedione-6-14C; 5-Fluoropyrimidine-2,4-dione-6-14C; 5-Fluoro-1H,3H-pyrimidine-2,4-dione-6-14C; NSC 19893-6-14C; Adrucil-6-14C (labeled analog); Efudex-6-14C (labeled analog); Fluorouracil-6-14C
EINECS	Contact for details

Quality Control

Every batch of 5-Fluorouracil-6-14C is manufactured and analyzed under strict quality control protocols to ensure identity, purity, and specific activity. Comprehensive analytical testing, including HPLC for chemical and radiochemical purity, NMR for structural confirmation, and mass spectrometry, is performed. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting the specific activity (mCi/mmol or GBq/mmol), radiochemical purity, chemical purity, and solvent information.

Storage

Preserve in a tightly closed container, protected from light. Store at recommended temperatures (typically 2-8°C or as specified on the COA). This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere if specified. Store in accordance with all local, national, and international regulations governing radioactive materials.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98% (Chemical)
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	50-60 mCi/mmol (1.85-2.22 GBq/mmol) or as customized
Solvent (for solutions)	Specified on COA (e.g., Ethanol, Water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.