Description

1,4-Diaminobutane-15N2 Dihydrochloride is a stable, isotopically labeled salt of putrescine, where both nitrogen atoms are enriched with the 15N isotope. This compound is essential as a high-purity internal standard and tracer for precise quantitative analysis in mass spectrometry-based metabolomic and proteomic research. It is primarily utilized by analytical laboratories, pharmaceutical R&D departments, and academic institutions engaged in advanced biochemical studies, pathway analysis, and drug metabolism investigations.

Application

• Internal Standard for LC-MS/MS: Used for the accurate quantification of endogenous putrescine and related polyamines in complex biological matrices like serum, urine, and tissue homogenates.
• Metabolic Pathway Tracer: Employed in stable isotope labeling experiments to elucidate the biosynthesis, turnover, and catabolic pathways of polyamines in cellular systems.
• Pharmaceutical Research: Serves as a critical reference material in drug discovery and development, particularly for studies involving polyamine metabolism as a therapeutic target.
• Biomarker Discovery: Facilitates the development and validation of robust analytical methods for identifying polyamine-based biomarkers in oncology and other disease states.
• Quality Control Reference: Acts as a certified reference material for calibrating instruments and ensuring the accuracy and reproducibility of analytical data in contract research organizations.
• Nutritional and Plant Science Studies: Applied in research investigating the role of polyamines in growth, development, and stress responses.

Basic Information

Product Name	1,4-Diaminobutane-15N2 Dihydrochloride
CAS No.	2747-92-4
Molecular Formula	C4H14Cl2N2 (15N2)
Molecular Weight	167.08 (for 15N2 labeled form)
Synonyms	Putrescine-15N2 dihydrochloride; 1,4-Butanediamine-15N2 dihydrochloride; Tetramethylenediamine-15N2 dihydrochloride; Butane-1,4-diamine-15N2 dihydrochloride; [15N2]-Putrescine dihydrochloride; 15N Labeled Putrescine 2HCl; Isotopic Putrescine Dihydrochloride
EINECS	Contact for details

Quality Control

Every batch of 1,4-Diaminobutane-15N2 Dihydrochloride is manufactured and analyzed under strict quality management protocols to ensure exceptional isotopic and chemical purity, critical for sensitive mass spectrometry applications. Our quality commitment includes comprehensive testing via NMR, HPLC, and MS to verify isotopic enrichment, chemical identity, and purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific data including assay, isotopic purity, and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere in a desiccator.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (15N)	≥ 98 atom % 15N
Isotopic Enrichment	≥ 99% on each labeled position
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.