Description

4β-Hydroxy Cholesterol-D4 (Major) is a high-purity, deuterium-labeled analog of a key oxysterol metabolite. This stable isotope-labeled compound is essential for precise quantitative analysis in biomedical research, serving as a critical internal standard to ensure analytical accuracy and reproducibility. It is primarily used by researchers and quality control laboratories in pharmaceutical development, clinical diagnostics, and metabolic studies focusing on cholesterol biosynthesis and related pathways.

Application

• Internal Standard for LC-MS/MS: Used as a deuterated internal standard for the accurate quantification of endogenous 4β-hydroxycholesterol in biological matrices via liquid chromatography-tandem mass spectrometry.
• Drug Metabolism & Pharmacokinetics (DMPK): Critical for studying the induction of cytochrome P450 3A4 (CYP3A4) activity, a key marker in drug-drug interaction studies.
• Clinical Research: Enables reliable measurement of 4β-hydroxycholesterol levels in patient plasma/serum for research into cholesterol homeostasis, liver function, and certain disease states.
• Biomarker Assay Development: Facilitates the development and validation of robust clinical assays for this important enzymatic activity biomarker.
• Pharmaceutical Quality Control: Supports analytical method development and validation in QC labs for drugs metabolized by the CYP3A4 pathway.
• Metabolomics & Lipidomics: A vital tool in targeted metabolomic profiling to understand perturbations in cholesterol metabolic pathways.

Basic Information

Product Name	4β-Hydroxy Cholesterol-D4 (Major)
CAS No.	1363529-44-5
Molecular Formula	C₂₇H₄₂D₄O₂
Molecular Weight	406.66 g/mol (approx.)
Synonyms	(22R)-4β-Hydroxycholesterol-25,26,26,26-d4; 4β-Hydroxycholesterol-d4; Cholest-5-ene-3β,4β-diol-25,26,26,26-d4; 4β-Hydroxy-5-cholesten-3β-ol-d4; Deuterated 4β-Hydroxycholesterol; 4β-OHC-d4; CYP3A4 Biomarker Internal Standard-d4; 25,26,26,26-tetradeutero-4β-hydroxycholesterol
EINECS	Contact for details

Quality Control

Every batch of 4β-Hydroxy Cholesterol-D4 (Major) is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, confirmed by advanced analytical techniques including NMR (¹H, ¹³C) and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing purity, isotopic enrichment, and solvent content. Our quality commitment ensures consistency and reliability for your most sensitive research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0% (Chemical Purity)
Isotopic Enrichment	≥ 98 atom % D
Isomeric Purity (4β-isomer)	≥ 95%
Solvent Content (Residual Solvents)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.