Description

7-(1-Methyl-2-Hydroxyethyl)Guanine-D7 (Major) is a stable isotope-labeled internal standard specifically designed for quantitative bioanalysis of guanine adducts in mass spectrometry-based assays. Its deuterium labeling (D7) ensures precise, interference-free quantification in complex biological matrices such as plasma, urine, and tissue homogenates. This compound is essential for researchers and contract research organizations (CROs) conducting DNA damage studies, biomarker validation, and regulatory toxicokinetic investigations. It supports high-sensitivity detection and accurate calibration in LC-MS/MS workflows across pharmaceutical development, academic research, and forensic toxicology laboratories.

Application

• Internal standard for LC-MS/MS quantification of 7-(1-methyl-2-hydroxyethyl)guanine adducts in biological samples
• Biomonitoring of ethyl carbamate (urethane) exposure and metabolic activation in human and animal studies
• Validation of analytical methods for DNA alkylation adducts per FDA/EMA bioanalytical method validation guidelines
• Supporting Good Laboratory Practice (GLP)-compliant toxicokinetic and genotoxicity studies
• Reference material for certified reference laboratories developing ISO/IEC 17025-accredited assays
• Calibration standard in clinical research labs investigating alcohol-related DNA damage pathways
• Quality control benchmark for commercial immunoassay kit development targeting guanine adducts

Basic Information

Product Name	7-(1-Methyl-2-Hydroxyethyl)Guanine-D7 (Major)
CAS No.	1346604-24-7
Molecular Formula	C9H11D7N5O2
Molecular Weight	232.25 g/mol
Synonyms	7-(1-(Methyl-d3)-2-hydroxyethyl)guanine-d4; 7-(1-Methyl-2-hydroxyethyl)guanine-13C,15N5; D7-7-(1-Methyl-2-hydroxyethyl)guanine; 7-(1-Methyl-2-hydroxyethyl)-[U-13C,15N5]guanine; 7-[1-(Methyl-d3)-2-hydroxyethyl]-[2,4,5,6,8-15N5]guanine; 7-(1-Methyl-2-hydroxyethyl)guanine-d7; N7-(1-Methyl-2-hydroxyethyl)guanine-d7; 7-(1-Methyl-2-hydroxyethyl)guanine-2,4,5,6,8-15N5-d3
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for isotopic enrichment (>98% D7), chemical purity (≥98.5% by HPLC-UV), identity (confirmed by LC-MS and 1H NMR), and absence of critical impurities (≤0.1% residual solvents, ≤0.05% heavy metals). Testing follows ICH Q2(R2) guidelines and aligns with ACS Reagent Grade specifications for isotopically labeled reference standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity and handle only in a controlled environment with desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (LC-MS)	Conforms to reference standard
Identification (NMR)	Conforms to reference spectrum
Assay (HPLC-UV)	≥98.5%
Isotopic Enrichment (D7)	≥98.0%
Residual Solvents (GC)	≤0.1% total
Water Content (KF)	≤0.5%
Heavy Metals (ICP-MS)	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.