Description

L-Alanine, n-[(S)-Hydroxyphenoxyphosphinyl]-, 1-Methylethyl Ester, (P→3'),(P'→5')-Diester With (2'R)-2'-Deoxy-2'-Fluoro-2'-Methyluridine is a sophisticated phosphoramidite monomer, a critical building block in oligonucleotide synthesis. This high-purity intermediate is essential for the automated solid-phase synthesis of therapeutic nucleic acids, enabling the precise incorporation of modified nucleosides. It is primarily utilized by pharmaceutical R&D teams and CDMOs developing advanced antisense oligonucleotides, siRNA, and other RNA-targeted therapeutics.

Application

• Phosphoramidite Monomer for Oligonucleotide Synthesis: Serves as a key raw material in automated DNA/RNA synthesizers for the production of modified oligonucleotides.
• Antisense Oligonucleotide (ASO) Development: Used to synthesize oligonucleotides for gene silencing and modulation in therapeutic applications.
• Small Interfering RNA (siRNA) Manufacturing: Critical for introducing the 2'-deoxy-2'-fluoro-2'-methyluridine modification, which enhances stability and potency.
• Research & Development of RNA Therapeutics: Enables academic and industrial labs to explore novel oligonucleotide sequences with improved pharmacokinetic properties.
• Contract Development and Manufacturing (CDMO): Supplied to service providers for the GMP and non-GMP production of clinical and commercial-stage nucleic acid drugs.
• Synthesis of Diagnostic Probes: Can be used to create labeled or modified probes for molecular diagnostics and research assays.

Basic Information

Product Name	L-Alanine, n-[(S)-Hydroxyphenoxyphosphinyl]-, 1-Methylethyl Ester, (P→3'),(P'→5')-Diester With (2'R)-2'-Deoxy-2'-Fluoro-2'-Methyluridine
CAS No.	1337482-17-3
Molecular Formula	C22H28FN4O9P
Molecular Weight	542.46 g/mol
Synonyms	2'-Deoxy-2'-fluoro-2'-methyluridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-2'-fluoro-2'-methyluridine 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-F-2'-Me-U Phosphoramidite; 2'-Fluoro-2'-methyluridine phosphoramidite; dF-MeU Phosphoramidite; 2'-Deoxy-2'-fluoro-2'-methyluridine CE Phosphoramidite; 5'-DMT-2'-F-2'-Me-U-CE Phosphoramidite
EINECS	Contact for details

Quality Control

This high-value pharmaceutical intermediate is produced under strict quality management systems. Each batch undergoes comprehensive analytical characterization using HPLC, NMR, and mass spectrometry to ensure identity, purity, and suitability for oligonucleotide synthesis. Certificates of Analysis (COA) detailing purity, moisture content, and solvent residues are provided with every shipment. Production can be aligned with GMP guidelines for advanced therapeutic applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term storage, maintain at -20°C in a dedicated freezer.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.