Description

L-Lysine-4,4,5,5-D4-Bis-n-T-Boc is a stable isotope-labeled derivative of the essential amino acid L-Lysine, where four hydrogen atoms are replaced by deuterium and the amine groups are protected by tert-butoxycarbonyl (Boc) groups. This compound is a critical tool in quantitative proteomics and metabolic research, enabling precise tracking and measurement of amino acid incorporation and protein turnover rates. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and CROs specializing in mass spectrometry-based biomarker discovery and pharmacokinetic studies.

Application

• Internal Standard for Mass Spectrometry (MS): Serves as a crucial quantitative internal standard in LC-MS/MS and GC-MS analyses for the accurate measurement of endogenous L-Lysine levels in biological samples.
• Proteomics & Metabolic Flux Analysis: Used in Stable Isotope Labeling by Amino acids in Cell culture (SILAC) and other flux studies to investigate protein synthesis, degradation, and cellular metabolism pathways.
• Pharmaceutical Development: Employed in drug metabolism and pharmacokinetics (DMPK) studies to trace the metabolic fate of drug candidates containing lysine moieties.
• Biomarker Research: Facilitates the discovery and validation of disease-specific biomarkers by allowing precise comparative quantification of proteins and metabolites.
• Synthetic Intermediate: Acts as a protected, labeled building block for the chemical synthesis of deuterated peptides, proteins, or other complex biomolecules for research purposes.
• Nutritional Science Studies: Used in research to study lysine absorption, utilization, and requirements in various biological systems.

Basic Information

Product Name	L-Lysine-4,4,5,5-D4-Bis-n-T-Boc
CAS No.	1314753-85-9
Molecular Formula	C₁₆H₂₆D₄N₂O₆
Molecular Weight	350.46 g/mol
Synonyms	N2,N6-Bis(tert-butoxycarbonyl)-L-lysine-4,4,5,5-d4; (2S)-2,6-Bis{[(2-methylpropan-2-yl)oxycarbonyl]amino}-6-aminohexanoic-4,4,5,5-d4 acid; Nα,Nε-Bis-Boc-L-lysine(D4); Boc-Lys(D4)-Boc; N2,N6-Di-Boc-L-lysine-4,4,5,5-d4; Deuterated Bis-Boc-L-Lysine; L-Lysine(D4) bis-Boc derivative; Isotopically labeled Boc-Lys(Boc)-OH
EINECS	Contact for details

Quality Control

Our L-Lysine-4,4,5,5-D4-Bis-n-T-Boc is manufactured under strict quality control protocols to ensure high isotopic purity and chemical integrity, essential for reliable research data. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (D%), chemical purity, and chiral purity. We adhere to relevant industry standards for fine chemicals and can provide material tailored to specific research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. Due to its hygroscopic nature, the container should be kept sealed in a desiccated environment to prevent moisture uptake, which can affect stability and handling. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment (D%)	≥98 atom % D
Optical Rotation	[α]²⁰D: Specific range provided on COA
Water Content (KF)	≤0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.