Description

Fmoc-L-Glutamic Acid-[13C5,15N]-5-T-Butyl Ester CAS NO 1261078-12-9 is a stable isotope-labeled derivative of the protected amino acid Fmoc-Glu(OtBu)-OH, where the carbon and nitrogen atoms are enriched with 13C and 15N isotopes. This compound is essential for advanced research in quantitative proteomics, metabolic pathway tracing, and pharmacokinetic studies, providing critical internal standards for mass spectrometry-based analysis. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and CROs specializing in biomarker discovery and drug development.

Application

• Mass Spectrometry Internal Standards: Used as a quantitative reference standard in LC-MS/MS and GC-MS for the accurate measurement of peptides and proteins.
• Metabolic Flux Analysis: Enables the tracing of glutamic acid metabolism in cell cultures and biological systems for metabolic engineering and disease research.
• Stable Isotope Labeling by Amino acids in Cell culture (SILAC): A key building block for synthesizing labeled peptides to study protein expression, turnover, and post-translational modifications.
• Pharmaceutical Development: Serves as a critical reagent in the synthesis of isotopically labeled drug candidates for ADME (Absorption, Distribution, Metabolism, and Excretion) studies.
• Proteomics Research: Facilitates the development of targeted and discovery-based proteomic assays to identify and quantify biomarkers.
• NMR Spectroscopy: The isotopic enrichment aids in structural elucidation and dynamic studies of biomolecules by nuclear magnetic resonance.

Basic Information

Product Name	Fmoc-L-Glutamic Acid-[13C5,15N]-5-T-Butyl Ester
CAS No.	1261078-12-9
Molecular Formula	C2613C5H2915N1O7
Molecular Weight	~466.5 g/mol (for labeled form)
Synonyms	Fmoc-Glu(OtBu)-OH-[13C5,15N]; N-(9-Fluorenylmethoxycarbonyl)-L-glutamic acid 5-tert-butyl ester-[13C5,15N]; (2S)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]amino]-5-(tert-butoxy)-5-oxopentanoic acid-[13C5,15N]; Fmoc-L-Glu(OtBu)-OH-[13C5,15N]; Fmoc-Glutamic acid 5-tert-butyl ester-[13C5,15N]; Isotopically labeled Fmoc-Glu(OtBu)-OH; Stable isotope-labeled Fmoc protected glutamic acid derivative
EINECS	Contact for details

Quality Control

Every batch of Fmoc-L-Glutamic Acid-[13C5,15N]-5-T-Butyl Ester is manufactured and tested under strict quality management systems. We guarantee high chemical purity and isotopic enrichment, supported by comprehensive analytical data including HPLC, NMR, and Mass Spectrometry. A Certificate of Analysis (COA) detailing purity, isotopic purity (atom % 13C and 15N), chiral purity, and other critical parameters is provided with each shipment to ensure compliance with your research and development protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (13C)	≥ 99 atom % 13C
Isotopic Purity (15N)	≥ 99 atom % 15N
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.