Description

5,6-Diamino-4-Hydroxypyrimidine-13C,15N is a stable isotope-labeled derivative of a key pyrimidine building block, essential for advanced analytical and research applications. This compound provides a critical internal standard for mass spectrometry, enabling precise quantification and metabolic pathway tracing in complex biological matrices. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in drug metabolism and pharmacokinetics (DMPK) studies, proteomics, and biomarker discovery.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, non-interfering reference compound for the accurate quantification of unlabeled 5,6-diamino-4-hydroxypyrimidine and related metabolites in biological samples.
• Drug Metabolism Studies (DMPK): Used in tracer experiments to elucidate metabolic pathways, bioavailability, and clearance mechanisms of pharmaceutical candidates.
• Proteomics & Biomarker Research: Facilitates stable isotope labeling by amino acids in cell culture (SILAC) or other techniques for comparative protein expression analysis.
• Nucleic Acid Research: Acts as a labeled precursor in the synthesis of isotopically enriched nucleosides or nucleotides for structural and mechanistic studies.
• Chemical Reference Standard: Provides a certified material for method development, validation, and quality control in analytical laboratories.
• Mechanistic Biochemistry: Enables detailed investigation of enzyme mechanisms involving pyrimidine scaffolds through isotope effect studies.

Basic Information

Product Name	5,6-Diamino-4-Hydroxypyrimidine-13C,15N
CAS No.	1246820-56-3
Molecular Formula	C413CH6N315NO
Molecular Weight	~130.08 g/mol (exact mass varies with isotopic enrichment)
Synonyms	4-Hydroxy-5,6-diaminopyrimidine-13C,15N; 5,6-Diamino-4(1H)-pyrimidinone-13C,15N; 5,6-Diamino-4-hydroxypyrimidine (13C,15N labeled); 5,6-Diamino-4-pyrimidinol-13C,15N; DAHP-13C,15N; Isotopically labeled 5,6-diaminouracil precursor; [13C,15N]-5,6-Diamino-4-hydroxypyrimidine
EINECS	Contact for details

Quality Control

Every batch of 5,6-Diamino-4-Hydroxypyrimidine-13C,15N is manufactured and analyzed under strict quality management systems. We guarantee high isotopic purity and chemical purity to meet the exacting standards of analytical research. Comprehensive Certificates of Analysis (COA) are provided, detailing isotopic enrichment (atom % 13C and 15N), chemical purity by HPLC, and identity confirmation.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant after opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (13C)	≥ 99 atom %
Isotopic Enrichment (15N)	≥ 99 atom %
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.