Description

24(R/S),25-Epoxycholesterol-D6 is a deuterated analog of a key oxysterol metabolite involved in cholesterol biosynthesis and signaling pathways. This high-purity, isotopically labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring accurate and reliable analytical results. It is primarily required by researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors for advanced metabolic studies, biomarker discovery, and drug development.

Application

• Internal Standard for LC-MS/MS: Critical for the precise quantification of endogenous 24,25-epoxycholesterol and related sterols in complex biological matrices like plasma, serum, and tissue homogenates.
• Metabolic Pathway Research: Used in tracer studies to investigate the intricacies of the cholesterol biosynthesis pathway, specifically the shunt pathway involving 24,25-epoxycholesterol.
• Biomarker Analysis: Enables the development and validation of sensitive assays for oxysterols, which are emerging biomarkers for diseases including atherosclerosis, neurodegenerative disorders, and cancer.
• Drug Discovery & Development: Supports pharmacokinetic and pharmacodynamic studies for drug candidates targeting cholesterol metabolism, liver X receptors (LXRs), or other nuclear receptors.
• Reference Material: Serves as a certified reference material (CRM) for calibrating analytical instruments and validating method accuracy in regulatory-compliant environments.

Basic Information

Product Name	24(R/S),25-Epoxycholesterol-D6
CAS No.	1246302-86-2
Molecular Formula	C27H38D6O2
Molecular Weight	406.66 g/mol (approx.)
Synonyms	(24R/S)-24,25-Epoxycholesterol-d6; 24,25-Epoxycholesterol-d6; 5-Cholestene-3β,24,25-triol-24,25-epoxide-d6; 24,25-Epoxy-5-cholestene-3β-ol-d6; 24,25-EC-d6; Deuterated 24,25-Epoxycholesterol; 24,25-Epoxycholesterol (hexadeuterated); 24,25-Epoxycholesterol Internal Standard
EINECS	Contact for details

Quality Control

Every batch of 24(R/S),25-Epoxycholesterol-D6 is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, verified by advanced analytical techniques including NMR, HPLC, and high-resolution mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment, and solvent content is provided with each shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic nature, the container should be allowed to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.