Description

4-Hydroxy Cholesterol-D7 is a high-purity, deuterium-labeled internal standard critical for advanced analytical chemistry. This compound is essential for achieving precise and accurate quantification in mass spectrometry-based assays, particularly for lipidomic and metabolic research. It is primarily utilized by pharmaceutical R&D laboratories, clinical research organizations, and academic institutions focusing on cholesterol metabolism, biomarker discovery, and drug development.

Application

• Internal Standard for LC-MS/MS: Used as a deuterated internal standard for the precise quantification of 4-hydroxy cholesterol and related oxysterols in complex biological matrices like plasma, serum, and tissue homogenates.
• Biomarker Research: Enables accurate measurement of 4-hydroxy cholesterol levels, a key biomarker associated with cholesterol metabolism, neurodegenerative diseases, and certain cancers.
• Drug Metabolism & Pharmacokinetics (DMPK): Facilitates studies on drug-induced changes in cholesterol biosynthesis and catabolic pathways.
• Stable Isotope Dilution Assays: Provides the reference point in high-sensitivity assays to correct for analyte loss during sample preparation and instrument variability.
• Clinical Diagnostics Development: Supports the development and validation of robust clinical diagnostic tests for inborn errors of metabolism and other disorders.
• Academic & Industrial Research: Serves as a critical reagent in fundamental research exploring the role of oxysterols in cell signaling, inflammation, and disease progression.

Basic Information

Item	Details
Product Name	4-Hydroxy Cholesterol-D7
CAS No.	1246302-80-6
Molecular Formula	C27H39D7O2
Molecular Weight	403.66 g/mol (approx.)
Synonyms	(25R)-26,26,26,27,27,27-Hexadeutero-3β-cholest-5-ene-3,26-diol; 26-Hydroxycholesterol-d7; 3β,26-Dihydroxycholest-5-ene-26,26,26,27,27,27-d7; 4-Hydroxycholesterol-d7; Cholest-5-ene-3β,26-diol-d7; 26-Hydroxy-3β-cholest-5-ene-d7; Deuterated 4-Hydroxy Cholesterol; Oxysterol-d7 Internal Standard
EINECS	Contact for details

Quality Control

Every batch of 4-Hydroxy Cholesterol-D7 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, supported by comprehensive analytical data including NMR, HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, isotopic enrichment, and absence of critical impurities. Our quality commitment ensures reliability for your most sensitive research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. For short-term use, store desiccated at 2-8°C. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (by NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.