Description

Orotic Acid-15N Monohydrate is a stable isotope-labeled derivative of orotic acid, a key intermediate in pyrimidine biosynthesis. This labeled compound is essential for advanced metabolic and pharmacokinetic studies, providing researchers with a precise tracer to elucidate biochemical pathways. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic manufacturers engaged in isotope dilution mass spectrometry and NMR spectroscopy.

Application

• Isotope-Labeled Internal Standard for the accurate quantification of orotic acid and related metabolites in biological matrices via LC-MS/MS.
• Metabolic Pathway Tracing in studies of pyrimidine synthesis, urea cycle disorders, and cellular nucleotide metabolism.
• Pharmaceutical Research for investigating drug mechanisms and conducting absorption, distribution, metabolism, and excretion (ADME) studies.
• Clinical Diagnostics as a critical component in assays for diagnosing hereditary orotic aciduria and other metabolic conditions.
• NMR Spectroscopy as a probe to study molecular structure, dynamics, and interactions without spectral interference.
• Nutritional Science Research to examine the role of orotic acid and nucleotide pools in health and disease states.

Basic Information

Product Name	Orotic Acid-15N Monohydrate
CAS No.	1229646-87-0
Molecular Formula	C5H415N2O4 • H2O
Molecular Weight	177.11 g/mol (for monohydrate)
Synonyms	6-Carboxyuracil-15N2 monohydrate; Vitamin B13-15N2 monohydrate; 1,2,3,6-Tetrahydro-2,6-dioxo-4-pyrimidinecarboxylic acid-15N2 monohydrate; Orotate-15N2 monohydrate; [15N2]-Orotic acid monohydrate; 6-Uracilcarboxylic acid-15N2 monohydrate; Wheeler-LaMer compound-15N2 monohydrate
EINECS	Contact for details

Quality Control

Our Orotic Acid-15N Monohydrate is manufactured under strict quality systems to ensure high chemical and isotopic purity suitable for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as assay, isotopic enrichment, and impurity profile. We adhere to relevant industry standards for research chemicals, ensuring reliable and reproducible performance in your laboratory.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Isotopic Enrichment (15N)	≥98 atom %
Water Content (KF)	7.0 - 10.0% (Theoretical for monohydrate: ~10.2%)
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.