Description

L-Glutamine-13C5, 15N2, D10 is a highly enriched, multi-isotope labeled analog of the essential amino acid L-glutamine. This compound is critical for advanced metabolic research, providing unparalleled precision for tracing pathways and quantifying protein and amino acid turnover. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and CROs specializing in mass spectrometry-based metabolomics and stable isotope-resolved studies.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to study central carbon metabolism, including the TCA cycle and glutaminolysis, in cell cultures and animal models.
• Quantitative Proteomics & Biomarker Discovery: Serves as an internal standard for the absolute quantification of glutamine and related metabolites in complex biological samples via LC-MS/MS.
• Drug Metabolism & Pharmacokinetics (DMPK): Employed in isotope dilution mass spectrometry to accurately determine the pharmacokinetic profiles of drug candidates affecting amino acid metabolism.
• Nutritional and Cancer Research: Investigates the role of glutamine metabolism in tumor cell proliferation, immune cell function, and cachexia.
• Stable Isotope Labeling by Amino acids in Cell culture (SILAC): Enables precise, multiplexed quantitative proteomics experiments in mammalian cell systems.
• NMR Spectroscopy Studies: Provides a high-sensitivity tracer for nuclear magnetic resonance studies of metabolic networks.

Basic Information

Product Name	L-Glutamine-13C5, 15N2, D10
CAS No.	1217443-24-7
Molecular Formula	¹³C₅H₁₀D₁₀¹⁵N₂O₃
Molecular Weight	187.22 g/mol
Synonyms	(2S)-2-Azaniumyl-4-carbamoyl-¹³C₅,1,1,2,2,3,3,4,4,4-D₁₀-butanoate-¹⁵N₂; L-Glutamine (13C5, 15N2, D10); L-Gln-13C5,15N2,D10; Glutamine-13C5,15N2,D10; (S)-2,5-Diamino-5-oxopentanoic-13C5,15N2,2,3,3,4,4,5,5,5-D8 acid; Isotopically labeled L-glutamine; Stable isotope-labeled glutamine; SILAC grade glutamine tracer.
EINECS	Contact for details

Quality Control

Every batch of L-Glutamine-13C5, 15N2, D10 is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Our products undergo rigorous quality testing, including NMR, HPLC, and HRMS, to ensure compliance with specifications for isotopic enrichment and chemical purity. Certificates of Analysis (COA) detailing isotopic purity (atom % 13C, 15N, D), chemical purity, and analytical methods are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation or storage under an inert atmosphere to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % 13C; ≥ 98 atom % 15N; ≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	+6.3° to +7.3° (c=2 in 1N HCl)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.