Description

Folic Acid-13C5 CAS NO 1207282-75-4 is a stable isotope-labeled analog of folic acid, where five carbon atoms are replaced with the Carbon-13 (13C) isotope. This compound is critically important as an internal standard for the precise and accurate quantification of folic acid in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is an essential tool for research and quality control laboratories in the pharmaceutical, clinical diagnostics, and nutritional science industries, ensuring reliable data for method development, validation, and regulatory compliance.

Application

• Internal Standard for Mass Spectrometry: Primary use as a quantitative reference standard in Liquid Chromatography-Mass Spectrometry (LC-MS/MS) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis of folic acid and its metabolites.
• Pharmaceutical Research & Development: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies to track the absorption, distribution, metabolism, and excretion of folic acid-based drugs.
• Clinical Diagnostics: Enables accurate measurement of folate levels in serum, plasma, and whole blood for the assessment of nutritional status and diagnosis of deficiencies.
• Nutritional Science & Food Analysis: Critical for the precise quantification of folic acid fortification in foods, dietary supplements, and infant formula to ensure label claim compliance.
• Metabolomics and Biomarker Discovery: Facilitates the study of folate-related metabolic pathways and the identification of novel biomarkers in disease research.
• Method Development and Validation: Serves as a key component in developing, optimizing, and validating robust analytical methods according to ICH, FDA, and other regulatory guidelines.
• Quality Control in API Manufacturing: Employed by active pharmaceutical ingredient (API) manufacturers to ensure the purity and potency of folic acid batches.

Basic Information

Product Name	Folic Acid-13C5
CAS No.	1207282-75-4
Molecular Formula	C1913C5H19N7O6
Molecular Weight	446.39 g/mol (approx.)
Synonyms	(6S)-2-[4-[(2-Amino-4-oxo-1,4-dihydropteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid-13C5; Pteroyl-13C5-L-glutamic acid; Vitamin B9-13C5; Folate-13C5; N-[4-[[(2-Amino-3,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-L-glutamic acid-13C5; [13C5]-Folic Acid
EINECS	Contact for details

Quality Control

Every batch of Folic Acid-13C5 is manufactured and analyzed under strict quality management systems. We guarantee high chemical purity and isotopic enrichment, with comprehensive testing including assay by HPLC, isotopic purity by MS, and identification by IR and NMR spectroscopy. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific results for all critical parameters to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions should be avoided to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 99 atom % 13C
Isotopic Enrichment	≥ 99% on five labeled positions
Chemical Purity	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.