Description

5-Methyltetrahydrofolic Acid-[13C5] is a stable, isotopically labeled form of the biologically active folate, 5-methyltetrahydrofolate (5-MTHF). This compound is essential for researchers and manufacturers requiring high-precision internal standards in quantitative mass spectrometry and metabolic studies. It is primarily utilized by analytical laboratories, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on folate metabolism, nutritional status assessment, and biomarker discovery.

Application

• Internal Standard for LC-MS/MS: Serves as a critical, high-purity reference standard for the accurate quantification of endogenous 5-methyltetrahydrofolate in biological matrices like plasma, serum, and tissue homogenates.
• Clinical Research & Diagnostics: Enables precise measurement of folate status in studies related to pregnancy, cardiovascular health, and neurological disorders, supporting the development of advanced diagnostic assays.
• Pharmaceutical Development: Used in pharmacokinetic (PK) and pharmacodynamic (PD) studies for novel folate-based therapeutics, ensuring accurate tracking of drug metabolites.
• Nutritional Science & Fortification Studies: Facilitates research into folate bioavailability, metabolism, and the efficacy of fortified foods and dietary supplements.
• Stable Isotope Tracer Studies: Acts as a tracer in metabolic flux analysis to map folate-dependent one-carbon metabolic pathways in cell culture and animal models.
• Quality Control & Method Validation: Essential for validating and calibrating analytical methods in GLP/GMP-compliant laboratories to ensure data integrity and regulatory compliance.

Basic Information

Item	Detail
Product Name	5-Methyltetrahydrofolic Acid-[13C5]
CAS No.	1207282-74-3
Molecular Formula	C20H20N7O6 (with five 13C atoms)
Molecular Weight	Contact for details
Synonyms	(6S)-5-Methyl-5,6,7,8-tetrahydrofolic Acid-[13C5]; L-Methylfolate-[13C5]; (6S)-5-MTHF-[13C5]; Metafolin-[13C5]; Levomefolic Acid-[13C5]; 5-Methyltetrahydrofolate-[13C5]; 5-Methyl-THF-[13C5]; 5-Methyl H4Folate-[13C5]
EINECS	Contact for details

Quality Control

Our 5-Methyltetrahydrofolic Acid-[13C5] is manufactured under strict quality systems to ensure the highest standards of purity and isotopic enrichment required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing HPLC purity, isotopic purity (enrichment), NMR confirmation, and residual solvent analysis. We adhere to relevant industry guidelines to guarantee product consistency and reliability for your critical research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (Enrichment)	≥ 98 atom % 13C
Chemical Purity	≥ 90%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.