Description

N6-(n-Threonylcarbonyl)Adenosine-13C4,15N is a stable isotope-labeled derivative of adenosine, a fundamental nucleoside in biochemistry. This compound, with its carbon-13 and nitrogen-15 atoms incorporated at specific positions, serves as a critical internal standard for precise quantitative analysis in mass spectrometry-based metabolomics and proteomics research. It is essential for researchers and manufacturers in the pharmaceutical, diagnostic, and life science sectors who require high-fidelity data for biomarker discovery, drug metabolism studies, and pathway analysis.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference standard in LC-MS/MS and GC-MS analyses for accurate measurement of adenosine and related metabolites in complex biological samples.
• Metabolomics Research: Enables precise tracking and quantification of purine metabolism pathways in studies of cellular energetics, signaling, and disease states like cancer or immune disorders.
• Pharmaceutical Development: Critical for pharmacokinetic (PK) and pharmacodynamic (PD) studies, allowing for the accurate determination of drug effects on nucleotide pools and related biochemical pathways.
• Diagnostic Assay Development: Employed in the calibration and validation of clinical diagnostic kits that measure adenosine or its derivatives as biomarkers for conditions such as ischemia or enzymatic deficiencies.
• Proteomics & Enzyme Activity Assays: Serves as a substrate or standard in assays investigating enzymes involved in adenosine modification, such as kinases and methyltransferases.
• Nutritional & Sports Science Research: Facilitates the study of ATP turnover, energy metabolism, and the role of supplements in athletic performance and recovery.

Basic Information

Product Name	N6-(n-Threonylcarbonyl)Adenosine-13C4,15N
CAS No.	1195030-28-4
Molecular Formula	C15H2113C415N5O8
Molecular Weight	~ 504.36 g/mol (for labeled form)
Synonyms	Adenosine, N-[N-[(1S,2R)-1-carboxy-2-hydroxypropyl]carbonyl]threonyl]-, (13C4,15N)-; N6-(L-Threonylcarbonyl)adenosine-13C4,15N; Isotopically labeled t6A; N6-Threonylcarbamoyladenosine-13C4,15N; (13C4,15N)-t6A; Labeled tRNA modification nucleoside; N6-(Threonylcarbonyl)adenosine-13C4,15N; Stable isotope-labeled adenosine derivative
EINECS	Contact for details

Quality Control

Every batch of N6-(n-Threonylcarbonyl)Adenosine-13C4,15N is manufactured and analyzed under strict quality systems to ensure identity, purity, and isotopic enrichment meet the exacting standards required for analytical applications. We provide comprehensive Certificates of Analysis (COA) detailing specifications for isotopic purity (e.g., 13C and 15N atom % excess), chemical purity by HPLC, and confirmation of structure. Our quality commitment aligns with the needs of GMP and ISO 9001 environments in research and manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, consider sealing under argon or nitrogen. Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (13C)	≥ 99 atom % 13C
Isotopic Purity (15N)	≥ 99 atom % 15N
Water Content (KF)	≤ 5.0 %
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.