Description

1-(2,5-Dioxopyrrolidin-1-Yloxy)-4-((5-Nitropyridin-2-Yl)Disulfanyl)-1-Oxobutane-2-Sulfonic Acid is a specialized heterobifunctional crosslinking reagent featuring a sulfosuccinimidyl ester (sulfo-NHS ester) and a pyridyl disulfide group. This compound is engineered for the efficient, site-specific conjugation of biomolecules, enabling the creation of stable, cleavable linkages under mild reducing conditions. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced proteomics, where precise and reversible protein modification is required.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a cleavable linker for attaching cytotoxic payloads to monoclonal antibodies via disulfide bonds.
• Protein/Peptide Labeling: Facilitates the site-specific introduction of labels, probes, or tags onto cysteine residues or other thiol-containing molecules.
• Surface Immobilization Chemistry: Used to functionalize biosensor chips, microarray slides, or nanoparticles with proteins for assay development.
• Polymer Conjugation: Enables the creation of smart polymer-drug conjugates with controlled release profiles.
• Biomaterial Modification: Applied in modifying hydrogels or other biomaterials to incorporate bioactive peptides or proteins.
• Diagnostic Reagent Synthesis: Key intermediate in the production of enzyme-linked or fluorescent immunoassay components.

Basic Information

Product Name	1-(2,5-Dioxopyrrolidin-1-Yloxy)-4-((5-Nitropyridin-2-Yl)Disulfanyl)-1-Oxobutane-2-Sulfonic Acid
CAS No.	1193111-37-3
Molecular Formula	C13H13N3O10S3
Molecular Weight	491.45 g/mol
Synonyms	Sulfo-NHS-SS-Pyridine; 5-Nitro-2-pyridyl 4-(2,5-dioxo-1-pyrrolidinyloxy)-4-oxo-2-sulfobutyl disulfide; 4-((5-Nitropyridin-2-yl)disulfanyl)-2-sulfobutyric acid 2,5-dioxopyrrolidin-1-yl ester; 2,5-Dioxopyrrolidin-1-yl 4-((5-nitropyridin-2-yl)disulfanyl)-2-sulfobutanoate; Sulfo-Succinimidyl 6-((5-Nitropyridin-2-yl)disulfanyl)hexanoate (common structural analog name); Heterobifunctional NHS-Pyridyl Disulfide Crosslinker; Sulfo-NHS ester-PySS crosslinker
EINECS	Contact for details

Quality Control

Our 1-(2,5-Dioxopyrrolidin-1-Yloxy)-4-((5-Nitropyridin-2-Yl)Disulfanyl)-1-Oxobutane-2-Sulfonic Acid is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the stringent requirements for bioconjugation research and cGMP-guided development. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and critical impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (by titration)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.