Description

5-Azacytosine-15N4 is a stable isotope-labeled analog of the nucleobase analog 5-azacytosine, where all four nitrogen atoms are replaced with the 15N isotope. This compound is essential for advanced research in nucleic acid chemistry and metabolism, serving as a critical internal standard for precise quantitative analysis using mass spectrometry. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, metabolomics, and isotope tracer studies.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference standard in LC-MS and GC-MS analysis for studying the pharmacokinetics and metabolism of 5-azacytosine and related compounds.
• Isotope Tracer Research: Enables the detailed tracking of nucleobase incorporation and metabolic pathways in biological systems for cancer and epigenetic research.
• Pharmaceutical R&D: Supports the development and quality control of novel therapeutics, including DNA methyltransferase inhibitors and antineoplastic agents.
• Metabolomics Studies: Facilitates accurate identification and quantification of cytosine analogs in complex biological samples.
• Chemical Synthesis Intermediate: Serves as a labeled building block for the synthesis of more complex isotopically-labeled nucleosides and nucleotides.
• Regulatory & Forensic Analysis: Provides a definitive standard for compliance testing and forensic investigations requiring high specificity.

Basic Information

Product Name	5-Azacytosine-15N4
CAS No.	1189895-68-8
Molecular Formula	C3H415N4O
Molecular Weight	116.06 g/mol (for 15N4)
Synonyms	4-Amino-1,3,5-triazin-2(1H)-one-15N4; 6-Aza-2-oxocytosine-15N4; 5-Azacytosine (15N4); 1,3,5-Triazin-2(1H)-one, 4-amino-15N4; Isotopically labeled 5-azacytosine; [15N4]-5-Azacytosine
EINECS	Contact for details

Quality Control

Our 5-Azacytosine-15N4 is manufactured under strict quality control protocols to ensure exceptional isotopic and chemical purity for research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and identity confirmation. We adhere to cGMP principles for consistent quality and traceability, meeting the stringent requirements of analytical and pharmaceutical laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed under an inert atmosphere after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Isotopic Enrichment (15N)	≥ 98 atom %
Assay (HPLC)	≥ 98.0%
Chemical Purity (HPLC)	≥ 99.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.