Description

1-Nicotinoyloxy-13C6 Succinimide is a stable isotope-labeled derivative of nicotinoyloxy succinimide, where the succinimide ring is uniformly labeled with six carbon-13 atoms. This compound serves as a critical reagent for the site-specific, stable isotope labeling of proteins and peptides, enabling advanced structural and functional studies in proteomics and biophysics. It is essential for researchers and manufacturers in pharmaceutical development, academic research, and contract research organizations (CROs) focused on mass spectrometry-based protein analysis, biomarker discovery, and drug-target interaction studies.

Application

• Isotope-Coded Affinity Tag (ICAT) Reagents: Used in quantitative proteomics for labeling cysteine residues in proteins for differential analysis via mass spectrometry.
• Chemical Cross-Linking Mass Spectrometry (XL-MS): Employed as a labeled cross-linker to determine protein-protein interactions and three-dimensional protein structures.
• Metabolic Pathway Tracing: Acts as a precursor or intermediate for synthesizing carbon-13 labeled NAD+ analogs to study cellular metabolism and enzyme mechanisms.
• Pharmaceutical Impurity Profiling: Utilized as an internal standard in LC-MS/MS methods for the accurate quantification of drug substances and related impurities.
• Bioconjugation and Protein Engineering: Facilitates the controlled introduction of nicotinamide or other functional groups onto biomolecules for creating novel conjugates.
• Stable Isotope Labeling by Amino acids in Cell culture (SILAC) Enhancement: Can be used to create specialized, chemically defined labeled media components or labeling reagents.

Basic Information

Product Name	1-Nicotinoyloxy-13C6 Succinimide
CAS No.	1173023-69-2
Molecular Formula	C1013C6H8N2O4
Molecular Weight	~274.19 (for 13C6 labeled form)
Synonyms	Nicotinoyloxy-13C6 Succinimide; 3-Carbamoyl-1-(pyridin-3-ylcarbonyloxy)-2,5-pyrrolidinedione-13C6; N-Succinimidyl Nicotinate-13C6; 13C6-Labeled NHS-Nicotinate; Isotope Labeled Nicotinoyloxy Succinimide Ester; 1-((Pyridine-3-carbonyl)oxy)-2,5-dihydro-1H-pyrrole-2,5-dione-13C6; 3-Pyridinecarboxylic Acid N-Hydroxysuccinimide Ester-13C6
EINECS	Contact for details

Quality Control

Our 1-Nicotinoyloxy-13C6 Succinimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high isotopic purity and chemical purity, critical for sensitive mass spectrometry applications. Certificates of Analysis (COA) are provided, detailing parameters such as isotopic enrichment, chemical purity by HPLC, and identity confirmation by NMR and MS. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed under inert atmosphere (argon or nitrogen) after each use to maintain purity and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (13C6)	≥ 99 atom % 13C
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.