Description

Guanosine-13C,15N2 Hydrate is a stable isotope-labeled nucleoside, specifically enriched with carbon-13 and nitrogen-15 at designated positions. This compound is essential as a critical internal standard and metabolic tracer in quantitative mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions engaged in advanced metabolomics, pharmacokinetic studies, and nucleic acid research.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a high-purity internal standard for the accurate quantification of guanosine and related metabolites in complex biological matrices.
• Metabolic Flux Analysis (MFA): Used as a tracer to study the purine salvage pathway, nucleotide synthesis, and cellular energy metabolism in cell cultures and in vivo models.
• NMR Spectroscopy: Provides a non-radioactive, stable isotope label for structural elucidation and dynamic studies of RNA and DNA molecules.
• Pharmaceutical Development: Critical for drug metabolism and pharmacokinetics (DMPK) studies to track the incorporation of nucleosides into newly synthesized nucleic acids.
• Clinical Research: Applied in biomarker discovery and validation studies related to mitochondrial disorders, cancer metabolism, and immune cell function.
• High-Purity Reference Standard: Used to calibrate analytical instruments and validate methods in quality control laboratories.

Basic Information

Item	Details
Product Name	Guanosine-13C,15N2 Hydrate
CAS No.	197227-95-5
Molecular Formula	C1013C H1315N2 N3 O5 · xH2O
Molecular Weight	Contact for details
Synonyms	9-β-D-Ribofuranosylguanine-13C,15N2 hydrate; 2-Amino-1,9-dihydro-9-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-6H-purin-6-one-13C,15N2 hydrate; Guanosine-13C10,15N2; [13C,15N2]-Guanosine; Isotopically Labeled Guanosine; Stable Isotope-Labeled Guanosine; Guanosine (13C,15N2)
EINECS	Contact for details

Quality Control

Every batch of Guanosine-13C,15N2 Hydrate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure exceptional isotopic and chemical purity. Our quality assurance protocol includes advanced techniques like HPLC, NMR, and mass spectrometry to verify identity, purity, and isotopic enrichment. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for isotopic purity, chemical purity, and residual solvents is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container should be sealed under an inert atmosphere after opening and allowed to equilibrate to room temperature before use to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 99 atom % 13C; ≥ 99 atom % 15N
Isotopic Enrichment (Positional)	Contact for details
Water Content (KF)	Contact for details
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.