Description

[2'-13C]Thymidine is a stable isotope-labeled nucleoside analog, specifically labeled with carbon-13 at the 2' position of the sugar moiety. This compound is a critical tool for advanced metabolic and pharmacokinetic studies, providing a non-radioactive tracer for precise quantification and pathway analysis. It is essential for researchers and manufacturers in the fields of pharmaceutical development, nuclear magnetic resonance (NMR) spectroscopy, and stable isotope probing (SIP) who require high-purity, isotopically enriched building blocks.

Application

• Metabolic Flux Analysis (MFA): Used as a tracer to study nucleotide metabolism, DNA synthesis rates, and cell proliferation in biological systems.
• Drug Metabolism and Pharmacokinetics (DMPK): Serves as an internal standard in LC-MS/MS for the accurate quantification of thymidine and its metabolites in bioanalytical studies.
• NMR Spectroscopy: Enables detailed structural and dynamic studies of nucleic acids and related enzymes due to the distinct NMR signal of the 13C label.
• Stable Isotope Probing (SIP): Tracks the incorporation of labeled nucleosides into DNA to identify and study active microbial communities in environmental samples.
• Biomedical Research: Investigates mechanisms in oncology, virology (e.g., antiviral drug development), and cellular biology related to DNA replication and repair.
• Synthesis of Labeled Compounds: Acts as a key precursor for the synthesis of more complex 13C-labeled nucleotides, oligonucleotides, or pharmaceutical agents.

Basic Information

Product Name	[2'-13C]Thymidine
CAS No.	185553-96-2
Molecular Formula	C10H1413CN2O5
Molecular Weight	Contact for details
Synonyms	1-(2-Deoxy-2-13C-β-D-erythro-pentofuranosyl)-5-methylpyrimidine-2,4(1H,3H)-dione; 2'-13C-Thymidine; Thymidine-2'-13C; 1-(2-Deoxy-2-13C-β-D-ribofuranosyl)thymine; dT-2'-13C; 2'-Deoxythymidine-2'-13C; 2'-13C-2'-Deoxythymidine; Labeled Thymidine
EINECS	Contact for details

Quality Control

Our [2'-13C]Thymidine is manufactured under strict quality systems to ensure exceptional isotopic and chemical purity for sensitive research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing parameters such as isotopic enrichment (Atom % 13C), chemical purity by HPLC, and identity confirmation. We adhere to relevant industry standards for stable isotope-labeled compounds to guarantee reliable and reproducible performance in your analytical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment (Atom % 13C)	≥99% at 2' position
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.